Denis-Magdelaine A, Flahault A, Verdy E
Service d'Hématologie Biologique, Hôpital Tenon, Paris, France.
Haemostasis. 1995 May-Jun;25(3):98-105. doi: 10.1159/000217148.
Activated partial thromboplastin time (APTT) is the most common assay used to screen for lupus anticoagulant (LA). For some years, manufacturers have been proposing APTT reagents that were more sensitive to LA. In this study, we evaluated the sensitivity and responsiveness of 16 APTT reagents in 40 LA and 46 normal plasmas. Sensitivity was established using a 1:1 mixture of normal and LA plasma that optimized the most sensitive reagent. The same pool of normal plasma was used for the assay of mixtures and daily APTT values for the determination of ratios. The results show that new commercially available APTT reagents are more sensitive and responsive to LA. The different sensitivities of APTT reagents might be partly due to the heterogeneity of LAs and several APTT reagents should be used for LA detection. The choice of APTT reagents constitutes an important preliminary step for LA evaluation.
活化部分凝血活酶时间（APTT）是用于筛查狼疮抗凝物（LA）最常用的检测方法。多年来，制造商一直在推出对LA更敏感的APTT试剂。在本研究中，我们评估了16种APTT试剂在40份LA血浆和46份正常血浆中的敏感性和反应性。使用正常血浆和LA血浆的1:1混合物确定敏感性，该混合物优化了最敏感的试剂。同一批正常血浆用于检测混合物以及测定比值的每日APTT值。结果表明，新的市售APTT试剂对LA更敏感且反应性更强。APTT试剂的不同敏感性可能部分归因于LA的异质性，并且应该使用几种APTT试剂来检测LA。APTT试剂的选择是LA评估的重要初步步骤。
