Kim Dongwoo, Chun Jae-Yong, Lee Kyeong-Yeoll
College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea.
Plant Quarantine Technology Center, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea.
Zookeys. 2016 Jun 22(600):1-5. doi: 10.3897/zookeys.600.8508. eCollection 2016.
At least 70 species of root-lesion nematodes, Pratylenchus spp., have been identified worldwide, many of which are serious pests of various agricultural crop plants. In Korea, only 14 species have been recorded in vegetable and fruit fields. Here, we report two new records of root-lesion nematode species in Korea based on morphometric and molecular methods. Soil samples were collected from chrysanthemum cultivars in various regions. Morphometric diagnosis showed that two new records for Korea: Pratylenchus kumamotoensis in Chilgok County and Pratylenchus pseudocoffeae in Geumsan County. In addition, molecular diagnosis using the two sequences of the internal transcribed spacer (ITS) and the D2-D3 region of ribosomal DNA showed that these two species were most similar with those from Japan, Costa Rica and USA. The similarities of the ITS and D2-D3 sequences were 99.85 and 99.74%, respectively, for Pratylenchus kumamotoensis and 99.99 with Costa Rica populations and 99.86% with USA populations, respectively, for Pratylenchus pseudocoffeae. To our knowledge, this is the first report of two species in Korea.
全世界已鉴定出至少70种根腐线虫（Pratylenchus spp.），其中许多是各种农作物的严重害虫。在韩国，蔬菜和果园中仅记录到14种。在此，我们基于形态学和分子方法报告韩国根腐线虫物种的两个新记录。从不同地区的菊花品种采集土壤样本。形态学诊断显示韩国有两个新记录：漆谷郡的熊本根腐线虫（Pratylenchus kumamotoensis）和锦山郡的拟咖啡根腐线虫（Pratylenchus pseudocoffeae）。此外，利用核糖体DNA的内转录间隔区（ITS）和D2 - D3区域的两个序列进行分子诊断表明，这两个物种与来自日本、哥斯达黎加和美国的物种最为相似。熊本根腐线虫的ITS和D2 - D3序列相似性分别为99.85%和99.74%，拟咖啡根腐线虫的ITS序列与哥斯达黎加种群的相似性为99.99%，与美国种群的相似性为99.86%。据我们所知，这是韩国首次报道这两个物种。
