Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China.
National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Environ Sci Pollut Res Int. 2016 Oct;23(19):19787-96. doi: 10.1007/s11356-016-7192-1. Epub 2016 Jul 14.
The present study was done to elucidate the effects of vanadium (V) on photosynthetic pigments, membrane damage, antioxidant enzymes, protein, and deoxyribonucleic acid (DNA) integrity in the following chickpea genotypes: C-44 (tolerant) and Balkasar (sensitive). Changes in these parameters were strikingly dependent on levels of V, at 60 and 120 mg V L(-1) induced DNA damage in Balkasar only, while photosynthetic pigments and protein were decreased from 15 to 120 mg V L(-1) and membrane was also damaged. It was shown that photosynthetic pigments and protein production declined from 15 to 120 mg V L(-1) and the membrane was also damaged, while DNA damage was not observed at any level of V stress in C-44. Moreover, the antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were increased in both genotypes of chickpea against V stress; however, more activities were observed in C-44 than Balkasar. The results suggest that DNA damage in sensitive genotypes can be triggered due to exposure of higher vanadium.
本研究旨在阐明钒（V）对以下鹰嘴豆基因型的光合色素、膜损伤、抗氧化酶、蛋白质和脱氧核糖核酸（DNA）完整性的影响：C-44（耐受）和 Balkasar（敏感）。这些参数的变化明显依赖于 V 的水平，在 60 和 120 mg V L(-1) 仅在 Balkasar 中诱导 DNA 损伤，而光合色素和蛋白质从 15 到 120 mg V L(-1) 减少，膜也受损。结果表明，在 C-44 中，光合色素和蛋白质的产生从 15 到 120 mg V L(-1)减少，膜也受损，但在任何 V 胁迫水平下都没有观察到 DNA 损伤。此外，两种鹰嘴豆基因型的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）等抗氧化酶活性均因 V 胁迫而增加；然而，C-44 中的活性高于 Balkasar。结果表明，由于暴露于较高的钒，敏感基因型可能会引发 DNA 损伤。
