Kasai Tomoko, Saegusa Sanae, Shirai Mitsuyuki, Murakami Masaru, Kato Yukio
Laboratory of Public Health 2.
Laboratory of Molecular Biology of Pharmacology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
Microbiol Immunol. 2016 Aug;60(8):540-51. doi: 10.1111/1348-0421.12401.
The incidence of MRSP has been increasing, and treatment options in veterinary medicine are limited. Few previous studies of MRSP have described the relationships between the genotypes, phenotypes, and clinical backgrounds of the isolates. To gain insight into the associations between the microbiological and clinical characteristics of MRSP, we analyzed 282 Staphylococcus pseudintermedius isolates from dogs. A total of 195 (69.1%) strains were identified as mecA-positive MRSP and were classified into mainly two genotypes: SCCmec types III (II-III) (52.8%) and V (37.4%). SCCmec type III MRSP strains were significantly correlated with hospital admission and antimicrobial therapy of the dogs, and exhibited a homogeneous genotype similar to sequence type 71-MRSP, which is a globally endemic clone in dogs. In contrast, SCCmec type V MRSP strains were not highly correlated with hospital admission and antimicrobial therapy and exhibited genotypic and phenotypic heterogeneity. Properties of MRSP strains SCCmec types III and V were similar to those of HA- and CA-MRSA, respectively. Therefore, we designated these isolates carrying SCCmec types III and V as HA-MRSP and CA-MRSP, respectively. Discrimination between HA- and CA-MRSP by oxacillin MIC will provide useful information for treatment and infection control measures for canine MRSP infections.
耐甲氧西林中间葡萄球菌（MRSP）的发病率一直在上升，而兽医学中的治疗选择有限。以前关于MRSP的研究很少描述分离株的基因型、表型和临床背景之间的关系。为了深入了解MRSP的微生物学特征与临床特征之间的关联，我们分析了282株来自犬的中间葡萄球菌分离株。总共195株（69.1%）菌株被鉴定为mecA阳性MRSP，并主要分为两种基因型：葡萄球菌盒式染色体 mec（SCCmec）III型（II-III）（52.8%）和V型（37.4%）。SCCmec III型MRSP菌株与犬的住院和抗菌治疗显著相关，并且表现出与序列型71-MRSP相似的同质基因型，序列型71-MRSP是犬类中的一种全球流行克隆。相比之下，SCCmec V型MRSP菌株与住院和抗菌治疗的相关性不高，并且表现出基因型和表型的异质性。MRSP菌株SCCmec III型和V型的特性分别与医院获得性耐甲氧西林金黄色葡萄球菌（HA-MRSA）和社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）相似。因此，我们将携带SCCmec III型和V型的这些分离株分别指定为HA-MRSP和CA-MRSP。通过苯唑西林最低抑菌浓度（MIC）区分HA-MRSP和CA-MRSP将为犬类MRSP感染的治疗和感染控制措施提供有用信息。
