Sofidiya Margaret O, Oloruntola Opeyemi M, Sofola Ikepo, Fageyinbo Muyiwa S
Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria.
Department of Pharmacology, Therapeutics and Toxicology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Nigeria.
J Tradit Complement Med. 2015 Sep 19;6(3):289-93. doi: 10.1016/j.jtcme.2015.07.006. eCollection 2016 Jul.
The leaves and roots of Euadenia trifoliolata are used in Nigeria traditional medicine for the treatment of ear ache, head ache and inflammation. The aim of the study was to evaluate the antinociceptive activity of ethanolic extract of the leaves (EL) and roots (ER) of E. trifoliolata in mice. Oral toxicity testing was performed using OECD guidelines. Antinociceptive effect was studied in mice using acetic acid-induced writhing, formalin, tail immersion and hot plate tests. Total polyphenolic contents were determined using standard methods. No mortality was recorded 24 h after oral administration of both EL and ER up to 5000 mg/kg. At the dose of 50, 100 and 200 mg/kg, administration of EL and ER resulted in significant reduction in the number of writhes compared to control. The percentage inhibition of writhings was calculated as 35.67%, 46.71% and 67.94% (EL) and 55.41%, 57.32% and 72.61% (ER), respectively. In hot plate test, EL and ER showed statistically significant antinociceptive effect, although low percentage inhibition (<50%) was recorded for ER at all the doses tested. Only EL (100 and 200 mg/kg) significantly (p < 0.001) increased the reaction time in tail immersion test. Both extracts significantly (p < 0.001) reduced the licking time in both phases of formalin test compared to control. The content of total phenolic, flavonoid and proanthocyanidin varies between the two extracts and may be the basis of the observed antinociceptive effect. The results indicate antinociceptive activity for the leaves and roots of E. trifoliolata, with the extract of the leaves showing better activity.
三叶尤阿登木的叶和根在尼日利亚传统医学中用于治疗耳痛、头痛和炎症。本研究的目的是评估三叶尤阿登木叶乙醇提取物（EL）和根乙醇提取物（ER）对小鼠的抗伤害感受活性。按照经济合作与发展组织（OECD）指南进行口服毒性试验。使用乙酸诱导扭体、福尔马林、尾浸和热板试验研究小鼠的抗伤害感受作用。采用标准方法测定总酚含量。口服EL和ER高达5000 mg/kg后24小时均未记录到死亡情况。在50、100和200 mg/kg剂量下，与对照组相比，给予EL和ER均导致扭体次数显著减少。扭体抑制率分别计算为35.67%、46.71%和67.94%（EL）以及55.41%、57.32%和72.61%（ER）。在热板试验中，EL和ER均显示出具有统计学意义的抗伤害感受作用，尽管在所有测试剂量下ER的抑制率均较低（<50%）。仅EL（100和200 mg/kg）在尾浸试验中显著（p < 0.001）延长了反应时间。与对照组相比，两种提取物在福尔马林试验的两个阶段均显著（p < 0.001）减少了舔舐时间。两种提取物中总酚、黄酮和原花青素的含量有所不同，这可能是观察到的抗伤害感受作用的基础。结果表明三叶尤阿登木的叶和根具有抗伤害感受活性，其中叶提取物显示出更好的活性。
