Gendrin Mathilde, Yerbanga Rakiswendé Serge, Ouedraogo Jean Bosco, Lefèvre Thierry, Cohuet Anna, Christophides George K
Department of Life Sciences , Imperial College London , United Kingdom.
Institut de Recherche en Sciences de la Santé , Bobo-Dioulasso , Burkina Faso.
Open Forum Infect Dis. 2016 Apr 13;3(2):ofw074. doi: 10.1093/ofid/ofw074. eCollection 2016 Apr.
Background. The gut microbiota of malaria vector mosquitoes grows after a blood meal and limits Plasmodium infection. We previously showed that penicillin and streptomycin in the ingested blood affect bacterial growth and positively impact mosquito survival and permissiveness to Plasmodium. In this study, we examine the effects of doxycycline, azithromycin, and co-trimoxazole. All 3 antibiotics are used in mass drug administration programs and have antimicrobial activities against bacteria and various stages of malaria parasites. Methods. The effects of blood meal supplementation with antibiotics on the mosquito microbiota, lifespan, and permissiveness to Plasmodium falciparum were assessed. Results. Ingestion of any of the 3 antibiotics significantly affected the mosquito microbiota. Azithromycin decreased P falciparum infection load and mosquito lifespan, whereas at high concentrations, doxycycline increased P falciparum infection load. Co-trimoxazole negatively impacted infection intensity but had no reproducible effect on mosquito lifespan. Conclusions. Our data suggest that the overall effect of antibiotic treatment on parameters critical for mosquito vectorial capacity is drug specific. The negative effect of azithromycin on malaria transmission is consistent with current efforts for disease elimination, whereas additional, larger scale investigations are required before conclusions can be drawn about doxycycline.
背景。疟疾传播媒介蚊子的肠道微生物群在吸食血液后会生长，并限制疟原虫感染。我们之前表明，摄入血液中的青霉素和链霉素会影响细菌生长，并对蚊子的存活以及对疟原虫的易感性产生积极影响。在本研究中，我们检测了强力霉素、阿奇霉素和复方新诺明的作用。这三种抗生素均用于大规模药物管理项目，并且对细菌和疟原虫的各个阶段均具有抗菌活性。方法。评估了在血液中添加抗生素对蚊子微生物群、寿命以及对恶性疟原虫易感性的影响。结果。摄入这三种抗生素中的任何一种都会显著影响蚊子的微生物群。阿奇霉素降低了恶性疟原虫的感染负荷和蚊子寿命，而在高浓度下，强力霉素会增加恶性疟原虫的感染负荷。复方新诺明对感染强度有负面影响，但对蚊子寿命没有可重复的影响。结论。我们的数据表明，抗生素治疗对蚊子传播能力的关键参数的总体影响因药物而异。阿奇霉素对疟疾传播的负面影响与当前的疾病消除努力一致，而在对强力霉素得出结论之前，还需要进行更多大规模的研究。
