Li Xu, Gao Aiai, Wang Yanan, Chen Man, Peng Jun, Yan Huaying, Zhao Xin, Feng Xizeng, Chen Dongyan
Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Department of Histology and Embryology, School of Medicine, Nankai University, Tianjin 300071, China.
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China.
Biol Open. 2016 Aug 15;5(8):1128-33. doi: 10.1242/bio.019497.
Maternal alcohol consumption during pregnancy can cause a series of developmental disorders in the fetus called FAS (fetal alcohol syndrome). In the present study we exposed zebrafish embryos to 1% and 2% alcohol and observed the morphology of heart and blood vessels during and after exposure to investigate motor function alterations, and damage and recovery to the cardiovascular system. The results showed that alcohol exposure could induce heart deformation, slower heart rate, and incomplete blood vessels and pericardium. After stopping exposure, larvae exposed to 1% alcohol could recover only in heart morphology, but larvae in 2% alcohol could not recover either morphology or function of cardiovascular system. The edema-like characteristics in the 2% alcohol group became more conspicuous afterwards, with destruction in the dorsal aorta, coarctation in segmental arteries and a decrease in motor function, implying more serious unrecoverable cardiovascular defects in the 2% group. The damaged blood vessels in the 2% alcohol group resulted in an alteration in permeability and a decrease of blood volume, which were the causes of edema in pathology. These findings contribute towards a better understanding of ethanol-induced cardiovascular abnormalities and co-syndrome in patients with FAS, and warns against excessive maternal alcohol consumption during pregnancy.
孕期母亲饮酒会导致胎儿出现一系列发育障碍，即胎儿酒精综合征（FAS）。在本研究中，我们将斑马鱼胚胎暴露于1%和2%的酒精中，并在暴露期间及之后观察心脏和血管的形态，以研究运动功能改变以及心血管系统的损伤和恢复情况。结果表明，酒精暴露可导致心脏变形、心率减慢以及血管和心包不完整。停止暴露后，暴露于1%酒精的幼虫仅在心脏形态上有所恢复，但暴露于2%酒精的幼虫在心血管系统的形态或功能上均未恢复。2%酒精组的水肿样特征随后变得更加明显，背主动脉遭到破坏，节段动脉出现缩窄，运动功能下降，这意味着2%酒精组存在更严重的不可恢复的心血管缺陷。2%酒精组受损的血管导致通透性改变和血容量减少，这是病理学上水肿的原因。这些发现有助于更好地理解乙醇诱导的心血管异常以及FAS患者的并发综合征，并警示孕期母亲不要过量饮酒。
