Li Jinshun, Xu Yuqin, Xu Jia, Wang Jinhua, Wu Liying
Department of Cardiology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150001, P.R. China.
Infectious Disease Department, Harbin Binghua Hospital, Harbin, Heilongjiang 150086, P.R. China.
Mol Med Rep. 2016 Sep;14(3):2045-51. doi: 10.3892/mmr.2016.5480. Epub 2016 Jul 7.
Venous congestion and volume overload are important in cardiorenal syndromes, in which multiple regulated factors are involved, including long non‑coding RNAs (lncRNAs). To investigate the underlying role of lncRNAs in regulating the development of venous congestion, an Affymetrix microarray associated with peripheral venous congestion was annotated, then a bipartite dynamic lncRNA-mRNA co-expression network was constructed in which nodes indicated lncRNAs or mRNAs. The nodes were connected when the lncRNAs or mRNAs were dynamically co‑expressed. Following functional analysis of this network, several dynamic alternative pathways were identified, including the calcium signaling pathway during venous congestion development. Additionally, certain lncRNAs (LINC00523, LINC01210 and RP11-435O5.5) were identified that may potentially dynamically regulate certain proteins, including plasma membrane calcium ATPase (PMCA) and G protein‑coupled receptor (GPCR), in the calcium signaling pathway. Particularly, the dynamically regulated switch of LINC00523 from co‑expression with PMCA to GPCR may be involved in damage to steady state intracellular calcium. In brief, the current study demonstrated a potential novel mechanism of lncRNA function during venous congestion.
静脉淤血和容量超负荷在心肾综合征中很重要，其中涉及多种调控因子，包括长链非编码RNA（lncRNA）。为了研究lncRNA在调节静脉淤血发展中的潜在作用，对与外周静脉淤血相关的Affymetrix微阵列进行注释，然后构建一个二分动态lncRNA-mRNA共表达网络，其中节点表示lncRNA或mRNA。当lncRNA或mRNA动态共表达时，节点相互连接。对该网络进行功能分析后，确定了几条动态替代途径，包括静脉淤血发展过程中的钙信号通路。此外，还鉴定出某些lncRNA（LINC00523、LINC01210和RP11-435O5.5），它们可能在钙信号通路中动态调节某些蛋白质，包括质膜钙ATP酶（PMCA）和G蛋白偶联受体（GPCR）。特别是，LINC00523从与PMCA共表达向GPCR的动态调节转换可能参与了细胞内钙稳态的破坏。简而言之，当前研究证明了静脉淤血期间lncRNA功能的一种潜在新机制。
