Chowdhary Anshika, Satagopam Venkata, Schneider Reinhard
Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.
Front Genet. 2021 Jul 1;12:649619. doi: 10.3389/fgene.2021.649619. eCollection 2021.
Long non-coding RNAs are diverse class of non-coding RNA molecules >200 base pairs of length having various functions like gene regulation, dosage compensation, epigenetic regulation. Dysregulation and genomic variations of several lncRNAs have been implicated in several diseases. Their tissue and developmental specific expression are contributing factors for them to be viable indicators of physiological states of the cells. Here we present an comprehensive review the molecular mechanisms and functions, state of the art experimental and computational pipelines and challenges involved in the identification and functional annotation of lncRNAs and their prospects as biomarkers. We also illustrate the application of co-expression networks on the TCGA-LIHC dataset for putative functional predictions of lncRNAs having a therapeutic potential in Hepatocellular carcinoma (HCC).
长链非编码RNA是一类多样的非编码RNA分子，长度大于200个碱基对，具有多种功能，如基因调控、剂量补偿、表观遗传调控。几种长链非编码RNA的失调和基因组变异与多种疾病有关。它们在组织和发育中的特异性表达是使其成为细胞生理状态可行指标的因素。在此，我们全面综述了长链非编码RNA的分子机制和功能、最新的实验和计算流程以及长链非编码RNA鉴定和功能注释中涉及的挑战及其作为生物标志物的前景。我们还阐述了共表达网络在TCGA-LIHC数据集上的应用，用于对在肝细胞癌（HCC）中具有治疗潜力的长链非编码RNA进行推定功能预测。
