Institute of Sciences of Food Production of the National Research Council of Italy (CNR-ISPA), Bari, 70126, Italy.
Department of Veterinary Medicine, University of Bari Aldo Moro, Valenzano, Bari, 70010, Italy.
Food Microbiol. 2020 Aug;89:103416. doi: 10.1016/j.fm.2020.103416. Epub 2020 Jan 10.
In this study the phenotypic and genomic characterization of two Arcobacter butzleri (Ab) strains (Ab 34_O and Ab 39_O) isolated from pre-cut ready-to-eat vegetables were performed. Results provided useful data about their taxonomy and their overall virulence potential with particular reference to the antibiotic and heavy metal susceptibility. These features were moreover compared with those of two Ab strains isolated from shellfish and a genotaxonomic assessment of the Ab species was performed. The two Ab isolated from vegetables were confirmed to belong to the Aliarcobacter butzleri species by 16S rRNA gene sequence analysis, MLST and genomic analyses. The genome-based taxonomic assessment of the Ab species brought to the light the possibility to define different subspecies reflecting the source of isolation, even though further genomes from different sources should be available to support this hypothesis. The strains isolated from vegetables in the same geographic area shared the same distribution of COGs with a prevalence of the cluster "inorganic ion transport and metabolism", consistent with the lithotrophic nature of Arcobacter spp. None of the Ab strains (from shellfish and from vegetables) metabolized carbohydrates but utilized organic acids and amino acids as carbon sources. The metabolic fingerprinting of Ab resulted less discriminatory than the genome-based approach. The Ab strains isolated from vegetables and those isolated from shellfish endowed multiple resistance to several antibiotics and heavy metals.
本研究对从预切即食蔬菜中分离得到的两株 Arcobacter butzleri（Ab）菌株（Ab 34_O 和 Ab 39_O）进行了表型和基因组特征分析。结果提供了有关其分类学和整体毒力潜力的有用数据，特别是在抗生素和重金属敏感性方面。这些特征还与从贝类中分离得到的两株 Ab 菌株进行了比较，并对 Ab 种进行了基因分类评估。通过 16S rRNA 基因序列分析、MLST 和基因组分析，证实从蔬菜中分离出的两株 Ab 菌株属于 Aliarcobacter butzleri 种。基于基因组的 Ab 种分类评估表明，有可能根据分离源定义不同的亚种，尽管需要来自不同来源的更多基因组来支持这一假设。在同一地理区域从蔬菜中分离出的菌株与同一 COG 分布共享，具有“无机离子运输和代谢”簇的普遍性，这与 Arcobacter spp 的自养性质一致。没有一株 Ab 菌株（来自贝类和蔬菜）代谢碳水化合物，但将有机酸和氨基酸用作碳源。Ab 的代谢指纹图谱分析不如基于基因组的方法具有区分力。从蔬菜和贝类中分离出的 Ab 菌株对多种抗生素和重金属具有多重耐药性。
