John Bincy Anu, Xu Tingting, Ripp Steven, Wang Hwa-Chain Robert
Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA.
Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN, USA.
Mol Imaging Biol. 2017 Feb;19(1):10-14. doi: 10.1007/s11307-016-0989-y.
The study was to develop an auto-bioluminescent urinary bladder cancer (UBC) xenograft animal model for pre-clinical research.
The study used a humanized, bacteria-originated lux reporter system consisting of six (luxCDABEfrp) genes to express components required for producing bioluminescent signals in human UBC J82, J82-Ras, and SW780 cells without exogenous substrates. Immune-deficient nude mice were inoculated with Lux-expressing UBC cells to develop auto-bioluminescent xenograft tumors that were monitored by imaging and physical examination.
Lux-expressing auto-bioluminescent J82-Lux, J82-Ras-Lux, and SW780-Lux cell lines were established. Xenograft tumors derived from tumorigenic Lux-expressing auto-bioluminescent J82-Ras-Lux cells allowed a serial, non-invasive, real-time monitoring by imaging of tumor development prior to the presence of palpable tumors in animals.
Using Lux-expressing auto-bioluminescent tumorigenic cells enabled us to monitor the entire course of xenograft tumor development through tumor cell implantation, adaptation, and growth to visible/palpable tumors in animals.
本研究旨在开发一种用于临床前研究的自动生物发光膀胱癌（UBC）异种移植动物模型。
本研究使用了一种人源化的、细菌起源的lux报告系统，该系统由六个（luxCDABEfrp）基因组成，可在无需外源底物的情况下，在人UBC J82、J82-Ras和SW780细胞中表达产生生物发光信号所需的成分。将表达Lux的UBC细胞接种到免疫缺陷裸鼠体内，以形成自动生物发光异种移植肿瘤，并通过成像和体格检查进行监测。
建立了表达Lux的自动生物发光J82-Lux、J82-Ras-Lux和SW780-Lux细胞系。源自致瘤性表达Lux的自动生物发光J82-Ras-Lux细胞的异种移植肿瘤，在动物出现可触及肿瘤之前，能够通过成像对肿瘤发展进行连续、非侵入性的实时监测。
使用表达Lux的自动生物发光致瘤细胞，使我们能够通过肿瘤细胞植入、适应和生长，直至在动物体内形成可见/可触及肿瘤，来监测异种移植肿瘤发展的全过程。
