Pais Maria Salomé
Academy of Sciences of Lisbon, Lisbon, Portugal.
Front Plant Sci. 2019 Mar 28;10:240. doi: 10.3389/fpls.2019.00240. eCollection 2019.
Very early somatic embryogenesis has been recognized as a powerful method to propagate plants . For some woody species and in particular for some coniferous trees, somatic embryogenesis induction has become a routine procedure. For the majority, the application of this technology presents yet many limitations especially due to the genotype, the induction conditions, the number of embryos produced, maturation, and conversion, among other factors that compromise the systematic use of somatic embryogenesis for commercial purposes especially of woody species and forest trees in particular. The advancements obtained on somatic embryogenesis in Arabidopsis and the development of OMIC technologies allowed the characterization of genes and the corresponding proteins that are conserved in woody species. This knowledge will help in understanding the molecular mechanisms underlying the complex regulatory networks that control somatic embryogenesis in woody plants. In this revision, we report on developments of OMICs (genomics, transcriptomics, metabolomics, and proteomics) applied to somatic embryogenesis induction and its contribution for understanding the change of fate giving rise to the expression of somatic embryogenesis competence.
极早期体细胞胚胎发生已被公认为一种强大的植物繁殖方法。对于一些木本物种，尤其是一些针叶树，体细胞胚胎发生诱导已成为常规程序。对于大多数物种而言，这项技术的应用仍存在许多局限性，特别是由于基因型、诱导条件、产生的胚胎数量、成熟度和转化率等因素，这些因素阻碍了体细胞胚胎发生技术在商业用途中的系统应用，尤其是在木本物种和森林树木方面。在拟南芥体细胞胚胎发生方面取得的进展以及组学技术的发展，使得能够鉴定在木本物种中保守的基因和相应蛋白质。这些知识将有助于理解控制木本植物体细胞胚胎发生的复杂调控网络背后的分子机制。在本综述中，我们报告了应用于体细胞胚胎发生诱导的组学（基因组学、转录组学、代谢组学和蛋白质组学）的发展及其对理解导致体细胞胚胎发生能力表达的命运变化的贡献。
