Alupei Liana, Peptu Catalina Anisoara, Lungan Andreea-Maria, Desbrieres Jacques, Chiscan Ovidiu, Radji Sadia, Popa Marcel
Technical University "Gheorghe Asachi", Faculty of Chemical Engineering and Protection of the Environment, Department of Natural and Synthetic Polymers, 73, Bd. Professor dr. docent Dimitrie Mangeron, 700050 Iasi, Romania.
Purolite SRL, Research & Development Department, Aleea Uzinei Street, No. 11, Victoria, Brasov, Romania.
Int J Biol Macromol. 2016 Nov;92:561-572. doi: 10.1016/j.ijbiomac.2016.07.058. Epub 2016 Jul 19.
The aim of the present study is to obtain, for the first time, polymer magnetic nanoparticles based on the chitosan-maltose derivative and magnetite. By chemically modifying the chitosan, its solubility in aqueous media was improved, which in turn facilitates the nanoparticles' preparation. Resulting polymers exhibit enhanced hydrophilia, which is an important factor in increasing the retention time of nanoparticles in the blood flow. The preparation of nanoparticles relied on the double crosslinking technique (ionic and covalent) in reverse emulsion which ensures the mechanical stability of the polymer carrier. The characterization of both the chitosan derivative and nanoparticles was accomplished by Fourier Transform Infrared Spectroscopy, Nuclear Magnetic Resonance Spectroscopy, Scanning Electron Microscopy, Transmission Electron Microscopy, Atomic Force Microscopy, Vibrating Sample Magnetometry, and Thermogravimetric Analysis. The evaluation of morphological, dimensional, structural, and magnetical properties, as well as thermal stability and swelling behavior of nanoparticles was made from the point of view of the polymer/magnetite ratio. The study of 5-Fluorouracil loading and release kinetics as well as evaluating the cytotoxicity and hemocompatibility of nanoparticles justify their adequate behavior in their potential use as devices for targeted transport of antitumor drugs.
本研究的目的是首次获得基于壳聚糖 - 麦芽糖衍生物和磁铁矿的聚合物磁性纳米颗粒。通过对壳聚糖进行化学修饰，提高了其在水性介质中的溶解度，这反过来又便于纳米颗粒的制备。所得聚合物表现出增强的亲水性，这是增加纳米颗粒在血流中保留时间的一个重要因素。纳米颗粒的制备依赖于反相乳液中的双重交联技术（离子交联和共价交联），这确保了聚合物载体的机械稳定性。壳聚糖衍生物和纳米颗粒的表征通过傅里叶变换红外光谱、核磁共振光谱、扫描电子显微镜、透射电子显微镜、原子力显微镜、振动样品磁强计和热重分析来完成。从聚合物/磁铁矿比例的角度对纳米颗粒的形态、尺寸、结构和磁性性质以及热稳定性和溶胀行为进行了评估。对5-氟尿嘧啶的负载和释放动力学的研究以及对纳米颗粒的细胞毒性和血液相容性的评估证明了它们在作为抗肿瘤药物靶向运输装置的潜在用途中具有适当的性能。
