Koeck Daniela E, Hahnke Sarah, Zverlov Vladimir V
Department of Microbiology, Technische Universität München, Emil-Ramann-Str. 4, D-85354 Freising-Weihenstephan, Germany.
Department of Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), D-14469 Potsdam, Germany.
Int J Syst Evol Microbiol. 2016 Oct;66(10):4132-4137. doi: 10.1099/ijsem.0.001324. Epub 2016 Jul 21.
Phylogenetic studies were performed on a group of novel Gram-stain-positive, anaerobic, non-sporulating rod-shaped bacteria isolated from a thermophilic biogas plant. The novel organisms were able to degrade crystalline cellulose. 16S rRNA gene sequence comparison indicated that the isolates SD1DT, SD1G, SD1I and RK1P share 100 % sequence identity, and are most closely related to Herbinix hemicellulosilytica T3/55T with which they share a 16S rRNA gene sequence similarity of 96.4 %. As a representative of the whole group of isolates, strain SD1DT was further characterized. Strain SD1DT was catalase-negative, indole-negative, and produced acetate, ethanol, butyric acid and hydrogen as major end-products during fermentative cellobiose utilization. Cells are rod-shaped, growing optimally at 40-65 °C and pH 6.5-8.5. The major cellular fatty acids (>10 %) were C19 : 0cyc 9,10 dimethyl acetal, C16 : 0 and C14 : 0. The DNA G+C content was 35.1 mol%. Due to the genetic and phenotypic differences to the most closely affiliated species, the isolates represent a novel species of the genus Herbinix within the family Lachnospiraceae, for which the name Herbinix luporum sp. nov. is proposed. The type strain is SD1DT(=DSM 100831T=CECT 8959T).
对从一个嗜热沼气厂分离出的一组新型革兰氏阳性、厌氧、无芽孢杆状细菌进行了系统发育研究。这些新型微生物能够降解结晶纤维素。16S rRNA基因序列比较表明，分离株SD1DT、SD1G、SD1I和RK1P的序列同一性为100%，与半纤维素解赫氏菌T3/55T关系最为密切，它们的16S rRNA基因序列相似性为96.4%。作为整个分离株组的代表，对SD1DT菌株进行了进一步表征。SD1DT菌株过氧化氢酶阴性、吲哚阴性，在利用纤维二糖发酵过程中产生乙酸、乙醇、丁酸和氢气作为主要终产物。细胞呈杆状，在40-65°C和pH 6.5-8.5条件下生长最佳。主要细胞脂肪酸（>10%）为C19:0环9,10二甲基缩醛、C16:0和C14:0。DNA G+C含量为35.1 mol%。由于与亲缘关系最密切的物种存在遗传和表型差异，这些分离株代表了毛螺菌科赫氏菌属的一个新物种，为此提出了新名称赫氏卢波菌（Herbinix luporum sp. nov.）。模式菌株为SD1DT（=DSM 100831T=CECT 8959T）。
