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通过RNA干扰敲低锌转运蛋白ZIP5可抑制食管癌的体内生长。

Knockdown of Zinc Transporter ZIP5 by RNA Interference Inhibits Esophageal Cancer Growth In Vivo.

作者信息

Li Qian, Jin Jing, Liu Jianghui, Wang Liqun, He Yutong

机构信息

Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

出版信息

Oncol Res. 2016;24(3):205-14. doi: 10.3727/096504016X14648701447896.

DOI:10.3727/096504016X14648701447896
PMID:27458102
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7838672/
Abstract

We recently found that SLC39A5 (ZIP5), a zinc transporter, is overexpressed in esophageal cancer. Downregulation of ZIP5 inhibited the proliferation, migration, and invasion of the esophageal cancer cell line KYSE170 in vitro. In this study, we found that downregulation of SLC39A5 (ZIP5) by interference resulted in a significant reduction in esophageal cancer tumor volume and weight in vivo. COX2 (cyclooxygenase 2) expression was decreased and E-cadherin expression was increased in the KYSE170K xenografts, which was caused by the downregulation of ZIP5. However, we did not find that the downregulation of ZIP5 caused a change in the relative expressions of cyclin D1, VEGF (vascular endothelial growth factor), MMP9 (matrix metalloprotein 9), and Bcl-2 (B-cell lymphoma/leukmia-2) mRNA or an alteration in the average level of zinc in the peripheral blood and xenografts in vivo. Collectively, these findings indicate that knocking down ZIP5 by small interfering RNA (siRNA) might be a novel treatment strategy for esophageal cancer with ZIP5 overexpression.

摘要

我们最近发现，锌转运蛋白SLC39A5（ZIP5）在食管癌中过表达。ZIP5的下调在体外抑制了食管癌细胞系KYSE170的增殖、迁移和侵袭。在本研究中，我们发现通过干扰下调SLC39A5（ZIP5）可导致体内食管癌肿瘤体积和重量显著降低。在KYSE170K异种移植瘤中，COX2（环氧化酶2）表达降低，E-钙黏蛋白表达增加，这是由ZIP5下调引起的。然而，我们没有发现ZIP5的下调导致细胞周期蛋白D1、VEGF（血管内皮生长因子）、MMP9（基质金属蛋白酶9）和Bcl-2（B细胞淋巴瘤/白血病-2）mRNA的相对表达发生变化，也没有发现体内外周血和异种移植瘤中锌的平均水平发生改变。总的来说，这些发现表明，用小干扰RNA（siRNA）敲低ZIP5可能是一种针对ZIP5过表达的食管癌的新型治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76a0/7838672/fe2737743afd/OR-24-205-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76a0/7838672/8c960fd7e49b/OR-24-205-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76a0/7838672/8c79a2efec63/OR-24-205-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76a0/7838672/405568901794/OR-24-205-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76a0/7838672/fe2737743afd/OR-24-205-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76a0/7838672/8c960fd7e49b/OR-24-205-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76a0/7838672/8c79a2efec63/OR-24-205-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76a0/7838672/405568901794/OR-24-205-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76a0/7838672/fe2737743afd/OR-24-205-g004.jpg

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