Schwenk J, Makovitzky J
Institute of Pathology, University of Erlangen, FRG.
Int J Pancreatol. 1989 Jul;5(1):85-98. doi: 10.1007/BF02925701.
The expression of the gastrointestinal cancer-associated antigens CA 19-9 and CA-50 was studied in 43 ductal pancreatic carcinomas, 1 mucinous cystadenoma, 1 signet-ring-cell carcinoma, 42 pancreata with chronic pancreatitis, and 10 normal fetal and adult pancreata. The anti-CA-50 antibody gave a more intense and more uniformly distributed staining of the ductal epithelial cells than the anti-CA 19-9 antibody. Both antigens, however, exhibited the same staining pattern of ductal epithelial cells in normal pancreas and chronic pancreatitis. Well differentiated carcinomas showed a predominantly membrane-bound antigen expression, whereas moderately and poorly differentiated carcinomas gave a more diffuse cytoplasmic staining. Epithelial dysplasia could not be differentiated by the staining pattern from normal, hyperplastic, metaplastic, or neoplastic cells. The immunohistochemical reaction with these anticarbohydrate antibodies, therefore, does not allow a qualitative discrimination between chronic pancreatitis and pancreatic carcinoma. CA 19-9, which expression depends on the Lewis gene, was negative in two patients with Le(a-b-) phenotype. Although anti-CA-50 antibody was reactive with the cancer cells of these 2 patients, the staining was weak and heterogenous.
对43例导管腺癌、1例黏液性囊腺瘤、1例印戒细胞癌、42例慢性胰腺炎胰腺以及10例正常胎儿和成人胰腺研究了胃肠道癌相关抗原CA 19-9和CA-50的表达。抗CA-50抗体比抗CA 19-9抗体对导管上皮细胞的染色更强且分布更均匀。然而，在正常胰腺和慢性胰腺炎中，两种抗原在导管上皮细胞上呈现相同的染色模式。高分化癌主要表现为膜结合抗原表达，而中分化和低分化癌则呈现更弥漫的胞质染色。上皮发育异常在染色模式上无法与正常、增生、化生或肿瘤细胞相区分。因此，用这些抗碳水化合物抗体进行的免疫组化反应无法在慢性胰腺炎和胰腺癌之间进行定性鉴别。CA 19-9的表达依赖于Lewis基因，2例Le(a-b-)表型患者的CA 19-9呈阴性。尽管抗CA-50抗体与这2例患者的癌细胞有反应，但染色较弱且不均匀。
