Guermani Enrico, Shaki Hossein, Mohanty Soumyaranjan, Mehrali Mehdi, Arpanaei Ayyoob, Gaharwar Akhilesh K, Dolatshahi-Pirouz Alireza
Department of Mechanical Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
Technical University of Denmark, DTU Nanotech, Center of Nanomedicine and Theranostics, Kongens Lyngby, 2800 Kgs. Lyngby, Region Hovedstaden, Denmark.
Sci Rep. 2016 Jul 28;6:30445. doi: 10.1038/srep30445.
Development of tissue engineering scaffolds with native-like biology and microarchitectures is a prerequisite for stem cell mediated generation of off-the-shelf-tissues. So far, the field of tissue engineering has not full-filled its grand potential of engineering such combinatorial scaffolds for engineering functional tissues. This is primarily due to the many challenges associated with finding the right microarchitectures and ECM compositions for optimal tissue regeneration. Here, we have developed a new microgel array to address this grand challenge through robotic printing of complex stem cell-laden microgel arrays. The developed microgel array platform consisted of various microgel environments that where composed of native-like cellular microarchitectures resembling vascularized and bone marrow tissue architectures. The feasibility of our array system was demonstrated through localized cell spreading and osteogenic differentiation of human mesenchymal stem cells (hMSCs) into complex tissue-like structures. In summary, we have developed a tissue-like microgel array for evaluating stem cell differentiation within complex and heterogeneous cell microenvironments. We anticipate that the developed platform will be used for high-throughput identification of combinatorial and native-like scaffolds for tissue engineering of functional organs.
开发具有天然生物学特性和微观结构的组织工程支架是干细胞介导生成即用型组织的先决条件。到目前为止，组织工程领域尚未充分发挥其设计此类组合支架以构建功能组织的巨大潜力。这主要是由于在寻找合适的微观结构和细胞外基质组成以实现最佳组织再生方面存在诸多挑战。在此，我们开发了一种新的微凝胶阵列，通过机器人打印复杂的负载干细胞的微凝胶阵列来应对这一巨大挑战。所开发的微凝胶阵列平台由各种微凝胶环境组成，这些环境由类似于血管化和骨髓组织结构的天然细胞微观结构构成。我们的阵列系统的可行性通过人骨髓间充质干细胞（hMSCs）在局部的细胞铺展和成骨分化形成复杂的组织样结构得到了证明。总之，我们开发了一种组织样微凝胶阵列，用于评估复杂和异质细胞微环境中的干细胞分化。我们预计，所开发的平台将用于高通量鉴定用于功能性器官组织工程的组合式和天然样支架。
