Weiss B, Sachs L
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1374-8. doi: 10.1073/pnas.75.3.1374.
Normal myeloid and MGI(+)D(+) clones of myeloid leukemic cells can be induced for Fc and complement component 3 rosettes, lysozme, and mature macrophages and granulocytes by a protein with macrophage- and granulocyte-inducing (MGI) activity, whereas MGI(+)D(-) clones can be induced by this protein for rosettes and lysozme but not mature cells. Lipopolysaccharides (LPS) from different bacteria induced the appearance of rosettes, lysozyme, and macrophages in some MGI(+)D(+) clones but did not induce any of these changes in MGI(+)D(-) clones. Lipid A gave the same results as LPS. Incubation of MGI(+)D(+) cells with LPS also induced an MGI activity detectable in the culture medium. This activity behaved like MGI in inducing (i) rosettes, lysozyme, and mature cells in MGI(+)D(+) leukemic cells including a clone resistant to LPS, (ii) rosettes and lysozyme in MGI(+)D(-) leukemic cells, and (iii) differentiation of normal myeloid cells to mature macrophages and granulocytes. This activity was induced in MGI(+)D(+) cells by LPS before the induction of rosettes or lysozyme. The results indicate that the lipid A portion of LPS indirectly induces differentiation of MGI(+)D(+) myeloid leukemic cells by inducing MGI protein. It is suggested that induction of specific regulatory proteins may be a more general mechanism for the induction of differentiation by surface-acting compounds.
具有巨噬细胞和粒细胞诱导（MGI）活性的蛋白质可诱导髓系白血病细胞的正常髓系和MGI(+)D(+)克隆形成Fc和补体成分3花环、溶菌酶，并分化为成熟巨噬细胞和粒细胞，而MGI(+)D(-)克隆可被该蛋白质诱导形成花环和产生溶菌酶，但不能诱导其分化为成熟细胞。来自不同细菌的脂多糖（LPS）可诱导一些MGI(+)D(+)克隆出现花环、溶菌酶和巨噬细胞，但不能诱导MGI(+)D(-)克隆发生任何这些变化。脂质A的结果与LPS相同。用LPS孵育MGI(+)D(+)细胞也可诱导培养基中出现可检测到的MGI活性。这种活性在诱导方面与MGI相似：（i）在MGI(+)D(+)白血病细胞（包括对LPS耐药的克隆）中诱导花环、溶菌酶和成熟细胞；（ii）在MGI(+)D(-)白血病细胞中诱导花环和溶菌酶；（iii）使正常髓系细胞分化为成熟巨噬细胞和粒细胞。在诱导花环或溶菌酶之前，LPS就在MGI(+)D(+)细胞中诱导了这种活性。结果表明，LPS的脂质A部分通过诱导MGI蛋白间接诱导MGI(+)D(+)髓系白血病细胞分化。有人提出，诱导特定调节蛋白可能是表面活性化合物诱导分化的更普遍机制。
