Manral Chetan, Roy Subhadeep, Singh Manjari, Gautam Swetlana, Yadav Rajnish K, Rawat Jitendra K, Devi Uma, Ansari Md Nazam, Saeedan Abdulaziz S, Kaithwas Gaurav
Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya vihar, Raibareli road, Lucknow, 226025, (U.P.), India.
Department of Pharmaceutical Sciences, FHMSIASM SHIATS-Deemed University (Formerly Allahabad Agriculture Institute), Naini, Allahabad, 211007, (U.P.), India.
BMC Complement Altern Med. 2016 Jul 29;16:260. doi: 10.1186/s12906-016-1243-5.
The present study was in quested to study the effects of β-sitosterol on methyl nitrosourea (MNU) induced mammary gland carcinoma in albino wistar rats.
Animals were randomized and divided into four groups of eight animals each. Group I (sham control 1 % CMC in normal saline p.o.); Group II (toxic control, MNU 47 mg/kg, i.v); Group III (MNU 47 mg/kg, i.v + β-sitosterol, 10 mg/kg, p.o); Group IV (MNU 47 mg/kg, i.v + β-sitosterol, 20 mg/kg, p.o). Toxicity was induced by single i.v. injection of MNU followed by β-sitosterol supplementation therapy for 115 days at the dose mentioned above.
Treatment with β-sitosterol evidenced decrease in the alveolar bud and lobule score in the whole mount of the mammary gland. β-sitosterol exhibited diminishing effect on oxidative stress through synchronizing lipid and enzymatic antioxidant defense. A significant decrease in the saturated and unsaturated fatty acid was evident with the MNU treatment and β-sitosterol demonstrated a marked effect on it. Pgp 9.5 expression was dose dependently upregulated by β-sitosterol treatment in comparison to MNU treatment. On the contrary, downregulated NF-kB expression was perceived, when β-sitosterol was concomitantly administered with MNU.
β-sitosterol afforded significant protection against the deleterious effects of MNU.
本研究旨在探讨β-谷甾醇对甲基亚硝基脲（MNU）诱导的白化Wistar大鼠乳腺癌的影响。
将动物随机分为四组，每组八只。第一组（假对照组，口服1%羧甲基纤维素钠生理盐水）；第二组（毒性对照组，静脉注射47mg/kg MNU）；第三组（静脉注射47mg/kg MNU + 口服10mg/kgβ-谷甾醇）；第四组（静脉注射47mg/kg MNU + 口服20mg/kgβ-谷甾醇）。通过单次静脉注射MNU诱导毒性，随后按上述剂量补充β-谷甾醇治疗115天。
β-谷甾醇治疗可使乳腺整体切片中的肺泡芽和小叶评分降低。β-谷甾醇通过同步脂质和酶抗氧化防御对氧化应激表现出减轻作用。MNU治疗后饱和脂肪酸和不饱和脂肪酸显著降低，β-谷甾醇对此有显著影响。与MNU治疗相比，β-谷甾醇治疗剂量依赖性地上调Pgp 9.5表达。相反，当β-谷甾醇与MNU同时给药时，可观察到NF-κB表达下调。
β-谷甾醇对MNU的有害作用提供了显著保护。
