Hodgson H J, Wands J R, Isselbacher K J
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1549-53. doi: 10.1073/pnas.75.3.1549.
We have studied peripheral blood mononuclear cells obtained from 24 patients with acute or chronic active hepatitis to determine if there was an abnormality in concanavalin A-induced suppressor cell activity compared to control subjects. Suppressor cells were generated by preincubation of the mononuclear cells with a mitogenic concentration of concanavalin A (6 mug/ml) for 48 hr followed by treatment with mitomycin C and alpha-methyl mannoside. Suppressor cell activity was assessed in second cultures by inhibition of concanavalin A-stimulated blast transformation of fresh allogeneic lymphocytes. Concanavalin A-stimulated suppressor activity was not elicited in mononuclear cells from the majority of patients with chronic active hepatitis in contrast to patients with acute hepatitis or acute inflammatory diseases and controls (P < 0.001). This finding was demonstrable in chronic active hepatitis patients in remission and relapse, both on and off prednisone therapy, and varied considerably during the course of the disease. The extent of liver injury was not related to the measured suppressor cell activity. These studies suggest that in chronic active hepatitis, a disease in which the host immune response may be involved, there appears to be a defect in concanavalin A-stimulated suppressor cells.
我们研究了从24例急性或慢性活动性肝炎患者获取的外周血单个核细胞，以确定与对照受试者相比，伴刀豆球蛋白A诱导的抑制细胞活性是否存在异常。通过将单个核细胞与促有丝分裂浓度的伴刀豆球蛋白A（6μg/ml）预孵育48小时，然后用丝裂霉素C和α-甲基甘露糖苷处理来产生抑制细胞。通过抑制伴刀豆球蛋白A刺激的新鲜同种异体淋巴细胞的母细胞转化，在二次培养中评估抑制细胞活性。与急性肝炎、急性炎症性疾病患者及对照相比，大多数慢性活动性肝炎患者的单个核细胞未诱导出伴刀豆球蛋白A刺激的抑制活性（P<0.001）。这一发现在缓解期和复发期的慢性活动性肝炎患者中均得到证实，无论是否接受泼尼松治疗，且在疾病过程中变化很大。肝损伤程度与测得的抑制细胞活性无关。这些研究表明，在慢性活动性肝炎这种可能涉及宿主免疫反应的疾病中，伴刀豆球蛋白A刺激的抑制细胞似乎存在缺陷。
