Holdstock G, Ershler W B, Krawitt E L
Gut. 1982 Sep;23(9):724-8. doi: 10.1136/gut.23.9.724.
Using a sensitive enzyme-linked immunosorbent assay technique to measure immunoglobulins produced by peripheral lymphocytes, patients with cirrhosis with, and without, hypergammaglobulinaemia were found to produce significantly more spontaneous IgG than controls (p less than 0.005). There was no difference in IgG production when pokeweed mitogen, a T-cell dependent B-cell mitogen, was added to the system. Contrary to our findings in a T-cell proliferative assay, there was no evidence of increased prostaglandin-producing suppressor cell activity in this system. To study the importance of B-cell stimulation in cirrhotic hyperglobulinaemia, normal mononuclear cells were exposed either to cirrhotic or to control sera before measuring spontaneous IgG production. Cells exposed to cirrhotic sera produced significantly more IgG than those exposed to the control sera (p less than 0.02). These findings suggest that non-specific B-cell activity occurs with patients in cirrhosis and it is argued that the severity of the hypergammaglobulinaemia in patients with cirrhosis is more likely to depend on the degree of non-specific B-cell stimulation than on the competence of the immunoregulatory system.
运用灵敏的酶联免疫吸附测定技术来检测外周淋巴细胞产生的免疫球蛋白，结果发现，患有高球蛋白血症和未患高球蛋白血症的肝硬化患者自发产生的IgG均显著多于对照组（p小于0.005）。当向系统中添加商陆有丝分裂原（一种T细胞依赖性B细胞有丝分裂原）时，IgG的产生没有差异。与我们在T细胞增殖试验中的发现相反，该系统中没有证据表明产生前列腺素的抑制细胞活性增加。为了研究B细胞刺激在肝硬化性高球蛋白血症中的重要性，在测量自发IgG产生之前，将正常单核细胞暴露于肝硬化患者血清或对照血清中。暴露于肝硬化患者血清的细胞产生的IgG明显多于暴露于对照血清的细胞（p小于0.02）。这些发现表明，肝硬化患者存在非特异性B细胞活性，并且有人认为，肝硬化患者高球蛋白血症的严重程度更可能取决于非特异性B细胞刺激的程度，而不是免疫调节系统的功能。
