Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Ataşehir, Istanbul 34755, Turkey.
Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Ataşehir, Istanbul 34755, Turkey.
Carbohydr Polym. 2016 Oct 20;151:313-320. doi: 10.1016/j.carbpol.2016.05.074. Epub 2016 May 24.
With their low toxicity, high mechanical strength and chemical stability, boron nitride nanotubes (BNNTs) are good candidates to enhance the properties of polymers, composites and scaffolds. Chitosan-based scaffolds are exhaustively investigated in tissue engineering because of their biocompatibility and antimicrobial activity. However, their spontaneous degradation prevents their use in a range of tissue engineering applications. In this study, hydroxylated BNNTs (BNNT-OH) were included into a chitosan scaffold and tested for their mechanical strength, swelling behavior and biodegradability. The results show that inclusion of BNNTs-OH into the chitosan scaffold increases the mechanical strength and pore size at values optimal for high cellular proliferation and adhesion. The chitosan/BNNT-OH scaffold was also found to be non-toxic to Human Dermal Fibroblast (HDF) cells due to its slow degradation rate. HDF cell proliferation and adhesion were increased as compared to the chitosan-only scaffold as observed by scanning electron microscopy (SEM) and fluorescent microscopy images.
氮化硼纳米管（BNNTs）具有低毒性、高强度和化学稳定性，是增强聚合物、复合材料和支架性能的理想选择。壳聚糖基支架在组织工程中得到了广泛的研究，因为它们具有生物相容性和抗菌活性。然而，它们的自发降解阻止了它们在一系列组织工程应用中的使用。在这项研究中，将羟基化的 BNNTs（BNNT-OH）掺入壳聚糖支架中，并对其机械强度、溶胀行为和生物降解性进行了测试。结果表明，BNNT-OH 的加入增加了壳聚糖支架的机械强度和孔径，达到了有利于高细胞增殖和黏附的最佳值。由于其缓慢的降解率，壳聚糖/BNNT-OH 支架对人真皮成纤维细胞（HDF）也表现出非毒性。通过扫描电子显微镜（SEM）和荧光显微镜图像观察到，与仅壳聚糖支架相比，HDF 细胞的增殖和黏附得到了增强。
