Kakarla Akesh Babu, Kong Ing, Kong Cin, Irving Helen, Thomas Colleen J
School of Computing, Engineering and Mathematical Sciences, La Trobe University, Bendigo, VIC 3552, Australia.
Department of Biomedical Sciences, Faculty of Science and Engineering, University of Nottingham Malaysia Campus, Semenyih 43500, Selangor, Malaysia.
Gels. 2022 Sep 21;8(10):603. doi: 10.3390/gels8100603.
Three-dimensional (3D) bioprinting, an innovative technology, has gained the attention of researchers as a promising technique for the redevelopment of complex tissue or organ structures. Despite significant advancements, a major challenge in 3D bioprinting is the limited number of suitable bioinks that fulfil the physiochemical requirements to produce complicated structures. Therefore, there is a demand for the production of bioinks for 3D bioprinting techniques. In this short communication, THP-1 cells encapsulated in boron nitride nanotubes (BNNTs) reinforced gelatin and alginate bioink was prepared. The study investigated the impact on the cells during printing using a fluorescence cell image. The results showed that the pure polymer bioinks demonstrated poor printability properties with the incorporation of cells. However, BNNT-combined bioink showed a significant increase in structural integrity even after the incorporation of cells. Furthermore, the scaffold structure was successfully printed with the cells incorporated bioink, and a considerable number of live cells were observed. With further studies, BNNTs as a promising nanomaterial for formulating bioink encapsulated with cells can be understood fully.
三维（3D）生物打印作为一种创新技术，作为一种用于重建复杂组织或器官结构的有前途的技术，已引起研究人员的关注。尽管取得了重大进展，但3D生物打印中的一个主要挑战是满足生产复杂结构的物理化学要求的合适生物墨水数量有限。因此，对用于3D生物打印技术的生物墨水的生产有需求。在本简短通讯中，制备了封装在氮化硼纳米管（BNNTs）增强的明胶和藻酸盐生物墨水中的THP-1细胞。该研究使用荧光细胞图像研究了打印过程中对细胞的影响。结果表明，纯聚合物生物墨水在加入细胞后表现出较差的可打印性。然而，即使加入细胞后，BNNT复合生物墨水的结构完整性也显著提高。此外，成功地用含有细胞的生物墨水打印了支架结构，并观察到大量活细胞。通过进一步研究，可以充分了解BNNTs作为一种用于配制封装细胞的生物墨水的有前途的纳米材料。
