Nass G, Poralla K
Mol Gen Genet. 1976 Aug 10;147(1):39-43. doi: 10.1007/BF00337933.
Twenty-two borrelidin resistant mutants of Saccharomyces cerivisiae were isolated, studied genetically and their threonyl-tRNA-synthetase was investigated. The borrelidin resistant mutants are classified into four groups. In the first group borrelidin resistance is coupled to the gene HOM3 coding for aspartokinase, in the second group to the gene LEU1. The borrelidin resistance in group three and four is not coupled to anyone of the genetic markers tested. Borrelidin resistance exhibited dominant behavior in all mutants except in the mutant of group 4. The properties of the ThrRS of the mutants of group one, two and four was found to be like the ones of the wild types. However the mutants of group three exhibit a structurally altered ThrRS, which is no longer inhibited by borrelidin.
分离出22株酿酒酵母的硼缬氨酸抗性突变体，对其进行了遗传学研究，并对它们的苏氨酰 - tRNA合成酶进行了研究。硼缬氨酸抗性突变体分为四组。在第一组中，硼缬氨酸抗性与编码天冬氨酸激酶的HOM3基因相关联；在第二组中，与LEU1基因相关联。第三组和第四组中的硼缬氨酸抗性与所测试的任何遗传标记均无关联。除了第四组的突变体外，硼缬氨酸抗性在所有突变体中均表现出显性特征。发现第一组、第二组和第四组突变体的苏氨酰 - tRNA合成酶的特性与野生型的相似。然而，第三组的突变体表现出结构改变的苏氨酰 - tRNA合成酶，该酶不再受硼缬氨酸抑制。
