Kappe R
Mycoses. 1989 Jan;32(1):24-32. doi: 10.1111/j.1439-0507.1989.tb02165.x.
Two commercially available latex agglutination tests: Cand-TEC(TM) from RAMCO Laboratories, Inc., Houston, Texas, USA, for the detection of a heat labile Candida antigen, and LA-Candida Antigen Detection System from Immuno-Mycologics (IMMY), Inc., Norman, Oklahoma, USA, for the detection of Candida mannan antigen, and Own LAT, a self-prepared latex agglutination test for the detection of Candida mannan antigen were tested on 467 sera of patients at risk for deep-seated Candidosis. Separate, parallel investigations were made to demonstrate anti-Candida antibodies (3 methods), circulating immune complexes (not antigen-specific, 2 methods), and rheumatoid factor (2 methods). Specificity and sensitivity of the mannan antigen detection systems were studied. Conditions of specific and non-specific mannan binding to antisera were studied in a defined mannan-antiserum system. The potential and the limitations of the Candida mannan antigen and protein antigen detection systems using latex particle agglutination are evaluated.
一种是美国得克萨斯州休斯顿市RAMCO实验室公司生产的Cand - TEC(TM)，用于检测一种热不稳定念珠菌抗原；另一种是美国俄克拉何马州诺曼市免疫真菌学（IMMY）公司生产的LA - 念珠菌抗原检测系统，用于检测念珠菌甘露聚糖抗原；还有自行制备的用于检测念珠菌甘露聚糖抗原的乳胶凝集试验（Own LAT），对467例有深部念珠菌病风险患者的血清进行了检测。进行了单独的平行研究以检测抗念珠菌抗体（3种方法）、循环免疫复合物（非抗原特异性，2种方法）和类风湿因子（2种方法）。研究了甘露聚糖抗原检测系统的特异性和敏感性。在一个明确的甘露聚糖 - 抗血清系统中研究了特异性和非特异性甘露聚糖与抗血清结合的条件。评估了使用乳胶颗粒凝集法检测念珠菌甘露聚糖抗原和蛋白质抗原系统的潜力和局限性。
