Yuen Po Ki
Science and Technology, Corning Incorporated , Corning, New York 14831-0001, USA.
Biomicrofluidics. 2016 Jul 13;10(4):044104. doi: 10.1063/1.4958909. eCollection 2016 Jul.
A novel method for integrating and embedding objects to add new functionalities during 3D printing based on fused deposition modeling (FDM) (also known as fused filament fabrication or molten polymer deposition) is presented. Unlike typical 3D printing, FDM-based 3D printing could allow objects to be integrated and embedded during 3D printing and the FDM-based 3D printed devices do not typically require any post-processing and finishing. Thus, various fluidic devices with integrated glass cover slips or polystyrene films with and without an embedded porous membrane, and optical devices with embedded Corning(®) Fibrance™ Light-Diffusing Fiber were 3D printed to demonstrate the versatility of the FDM-based 3D printing and embedding method. Fluid perfusion flow experiments with a blue colored food dye solution were used to visually confirm fluid flow and/or fluid perfusion through the embedded porous membrane in the 3D printed fluidic devices. Similar to typical 3D printed devices, FDM-based 3D printed devices are translucent at best unless post-polishing is performed and optical transparency is highly desirable in any fluidic devices; integrated glass cover slips or polystyrene films would provide a perfect optical transparent window for observation and visualization. In addition, they also provide a compatible flat smooth surface for biological or biomolecular applications. The 3D printed fluidic devices with an embedded porous membrane are applicable to biological or chemical applications such as continuous perfusion cell culture or biocatalytic synthesis but without the need for any post-device assembly and finishing. The 3D printed devices with embedded Corning(®) Fibrance™ Light-Diffusing Fiber would have applications in display, illumination, or optical applications. Furthermore, the FDM-based 3D printing and embedding method could also be utilized to print casting molds with an integrated glass bottom for polydimethylsiloxane (PDMS) device replication. These 3D printed glass bottom casting molds would result in PDMS replicas with a flat smooth bottom surface for better bonding and adhesion.
本文提出了一种基于熔融沉积建模（FDM）（也称为熔丝制造或熔融聚合物沉积）在3D打印过程中集成和嵌入物体以添加新功能的新方法。与典型的3D打印不同，基于FDM的3D打印可以在3D打印过程中集成和嵌入物体，并且基于FDM的3D打印设备通常不需要任何后处理和精加工。因此，3D打印了各种集成玻璃盖玻片或带有和不带有嵌入式多孔膜的聚苯乙烯薄膜的流体装置，以及嵌入了康宁（®）Fibrance™光扩散纤维的光学装置，以证明基于FDM的3D打印和嵌入方法的多功能性。使用蓝色食用染料溶液进行流体灌注流动实验，以直观地确认流体通过3D打印流体装置中嵌入式多孔膜的流动和/或流体灌注。与典型的3D打印设备类似，基于FDM的3D打印设备充其量是半透明的，除非进行后抛光，并且在任何流体装置中都非常需要光学透明度；集成玻璃盖玻片或聚苯乙烯薄膜将为观察和可视化提供完美的光学透明窗口。此外，它们还为生物或生物分子应用提供了兼容的平坦光滑表面。带有嵌入式多孔膜的3D打印流体装置适用于生物或化学应用，如连续灌注细胞培养或生物催化合成，但无需任何设备后组装和精加工。嵌入康宁（®）Fibrance™光扩散纤维的3D打印设备将在显示、照明或光学应用中得到应用。此外，基于FDM的3D打印和嵌入方法还可用于打印带有集成玻璃底部的铸模，用于聚二甲基硅氧烷（PDMS）设备复制。这些3D打印的玻璃底部铸模将产生具有平坦光滑底部表面的PDMS复制品，以实现更好的粘结和粘附。
