Loya Asif, Serrano Beatriz, Rasheed Farah, Tous Sara, Hassan Mariam, Clavero Omar, Raza Muhammad, De Sanjosé Silvia, Bosch F Xavier, Alemany Laia
Department of Pathology, Shaukat Khanum Memorial Cancer Hospital and Research Centre Lahore, Punjab 54000, Pakistan.
Unit of Infections and Cancer (UNIC), Cancer Epidemiology Research Programme, Catalan Institute of Oncology (ICO)-IDIBELL, L'Hospitalet de Llobregat, Gran Via de l'Hospitalet, 199-203, 08908 L'Hospitalet de Llobregat, Barcelona, Spain.
Cancers (Basel). 2016 Jul 30;8(8):72. doi: 10.3390/cancers8080072.
Few studies have assessed the burden of human papillomavirus (HPV) infection in Pakistan. We aim to provide specific information on HPV-type distribution in invasive cervical cancer (ICC) in the country. A total of 280 formalin-fixed paraffin-embedded tissue blocks were consecutively selected from Shaukat Khanum Memorial Cancer Hospital and Research Centre (Lahore, Pakistan). HPV-DNA was detected by SPF10 broad-spectrum PCR followed by DNA enzyme immunoassay and genotyping by LiPA25. HPV-DNA prevalence was 87.5% (95%CI: 83.0-91.1), with 96.1% of cases histologically classified as squamous cell carcinoma. Most of the HPV-DNA positive cases presented single infections (95.9%). HPV16 was the most common type followed by HPV18 and 45. Among HPV-DNA positive, a significantly higher contribution of HPV16/18 was detected in Pakistan (78.4%; 72.7-83.3), compared to Asia (71.6%; 69.9-73.4) and worldwide (70.8%; 69.9-71.8) and a lower contribution of HPVs31/33/45/52/58 (11.1%; 7.9-15.7 vs. 19.8%; 18.3-21.3 and 18.5%; 17.7-19.3). HPV18 or HPV45 positive ICC cases were significantly younger than cases infected by HPV16 (mean age: 43.3, 44.4, 50.5 years, respectively). A routine cervical cancer screening and HPV vaccination program does not yet exist in Pakistan; however, the country could benefit from national integrated efforts for cervical cancer prevention and control. Calculated estimations based on our results show that current HPV vaccine could potentially prevent new ICC cases.
很少有研究评估巴基斯坦人乳头瘤病毒（HPV）感染的负担。我们旨在提供该国浸润性宫颈癌（ICC）中HPV型别分布的具体信息。从巴基斯坦拉合尔的沙卡特汗姆纪念癌症医院及研究中心连续选取了280个福尔马林固定石蜡包埋组织块。通过SPF10广谱PCR检测HPV-DNA，随后进行DNA酶免疫测定，并通过LiPA25进行基因分型。HPV-DNA患病率为87.5%（95%置信区间：83.0 - 91.1），96.1%的病例组织学分类为鳞状细胞癌。大多数HPV-DNA阳性病例为单一感染（95.9%）。HPV16是最常见的类型，其次是HPV18和45。在HPV-DNA阳性病例中，与亚洲（71.6%；69.9 - 73.4）和全球（70.8%；69.9 - 71.8）相比，巴基斯坦HPV16/18的占比显著更高（78.4%；72.7 - 83.3），而HPV31/33/45/52/58的占比更低（11.1%；7.9 - 15.7，相比之下亚洲为19.8%；18.3 - 21.3，全球为18.5%；17.7 - 19.3）。HPV18或HPV45阳性的ICC病例明显比HPV16感染病例年轻（平均年龄分别为43.3岁、44.4岁、50.5岁）。巴基斯坦尚未开展常规宫颈癌筛查和HPV疫苗接种项目；然而，该国可从全国性的宫颈癌预防和控制综合努力中受益。根据我们的结果进行的估算表明，目前的HPV疫苗有可能预防新的ICC病例。
