Mogarekar Mukund Ramchandra, Dhabe Mahendra G, Gujrathi Chanchal C
Department of Biochemistry, Swami Ramanand Teerth Government Medical College, Ambajogai, 431517 Beed District, Maharashtra India.
J Obstet Gynaecol India. 2016 Oct;66(5):327-32. doi: 10.1007/s13224-015-0704-y. Epub 2015 May 16.
Aim of this study is to find out clinical relevance of estimating PON1 arylesterase activity, total oxidative stress (TOS), nitric oxide (NO), and vitamin C levels in maternal serum for prediction of birth weight of newborn.
We have investigated the PON1 arylesterase activity, TOS, NO, vitamin C, total protein, and albumin levels in 56 postnatal clinic patients having newborn weighing <2500 gm (low birth weight) and compared with 56 postnatal clinic patients having newborn weighing >2500 gm. Samples were collected immediately after delivery.
PON1 arylesterase activity levels show significant decrease in cases as compared to controls (93.27 ± 13.76 kU/l vs. 112.77 ± 9.42 kU/l). Nitric oxide (nitrate + nitrite) levels are also found to be significantly decreased in cases with respect to controls (22.89 ± 2.65 umol/l vs. 24.73 ± 3.80 umol/l). Total oxidative stress is significantly increased in cases than in control subjects (23.34 ± 2.64 μmol H2O2 equiv./l vs. ± 21.43 ± 2.47 μmol H2O2 equiv/l). Vitamin C levels are also significantly decreased in cases as compared to controls (1.23 ± 0.25 mg/dl vs. 1.34 ± 0.28 mg/dl). Positive correlation between neonatal birth weight and maternal serum PON1 arylesterase activity (r = 0.682, p < 0.05) while negative correlation is obtained between neonatal birth weight and maternal serum oxidative stress (r = -0.478, p < 0.05). Logistic regression analysis is applied for assessing predictive utility which demonstrated a significant association of birth weight with PON1 arylesterase activity (AUC = 0.960, Naglekerke's R (2) = 0.793, p < 0.05).
Decreased arylesterase activity and antioxidant vitamin C levels with increased total oxidative stress in maternal serum may be considered as the additional risk factors for the development of low birth weight newborn.
本研究旨在探讨测定母血中对氧磷酶1（PON1）芳基酯酶活性、总氧化应激（TOS）、一氧化氮（NO）和维生素C水平对预测新生儿出生体重的临床意义。
我们对56例产后门诊新生儿体重<2500克（低出生体重）的患者进行了PON1芳基酯酶活性、TOS、NO、维生素C、总蛋白和白蛋白水平的检测，并与56例产后门诊新生儿体重>2500克的患者进行了比较。样本在分娩后立即采集。
与对照组相比，病例组的PON1芳基酯酶活性水平显著降低（93.27±13.76kU/l对112.77±9.42kU/l）。病例组的一氧化氮（硝酸盐+亚硝酸盐）水平也显著低于对照组（22.89±2.65μmol/l对24.73±3.80μmol/l）。病例组的总氧化应激显著高于对照组（23.34±2.64μmol H2O2当量/升对21.43±2.47μmol H2O2当量/升）。与对照组相比，病例组的维生素C水平也显著降低（1.23±0.25mg/dl对1.34±0.28mg/dl）。新生儿出生体重与母血PON1芳基酯酶活性呈正相关（r=0.682，p<0.05），而新生儿出生体重与母血氧化应激呈负相关（r=-0.478，p<0.05）。应用逻辑回归分析评估预测效用，结果显示出生体重与PON1芳基酯酶活性显著相关（AUC=0.960，Naglekerke's R(2)=0.793，p<0.05）。
母血中芳基酯酶活性降低、抗氧化维生素C水平降低以及总氧化应激增加可能被视为低出生体重新生儿发生的额外危险因素。
