Asjid Rafia, Faisal Tayyaba, Qamar Khadija, Khan Saleem Ahmed, Khalil Aamna, Zia Muhammad Sarwar
Anatomy, Army Medical College, Rawalpindi, PAK.
Haematology, Army Medical College, Rawalpindi, PAK.
Cureus. 2019 Nov 1;11(11):e6050. doi: 10.7759/cureus.6050.
The search for minimally invasive treatment of osteoarthritis has led to the development of biological options such as platelet-rich plasma (PRP), mesenchymal stem cells (MSCs), and bone marrow aspirate concentrates. This research was conducted to study the outcomes of PRP administration in the chemical-induced model of osteoarthritis in rat knee. Methods and results Two milligrams of monoiodoacetate (MIA) was used for the induction of arthritis in the right knee of 16 rats. Autologous PRP was prepared by double centrifugation, which was then administered in the arthritic knee of eight rats. This group was labeled as the treated group (A) while the rest were counted as the non-treated group (B). Chondrocyte count and uncalcified cartilage thickness were morphometrically assessed on hematoxylin and eosin (H&E) stained slides, and it was noted that treated group A had a higher chondrocyte count and more cartilage height as compared to non-treated group B. Intergroup comparison was done between the treated group (A) and non-treated group (B) using the independent t-test. P-values were found to be statistically significant for these parameters. Conclusion This study thus concluded that PRP had induced an inhibitory effect on the apoptosis of chondrocytes, which, in turn, prevented the loss of cartilage height by inhibiting matrix loss.
对骨关节炎微创治疗方法的探索促使了生物治疗方案的发展，如富血小板血浆（PRP）、间充质干细胞（MSCs）和骨髓抽吸浓缩物。本研究旨在探讨PRP注射对大鼠膝关节化学诱导性骨关节炎模型的治疗效果。方法与结果：采用2mg单碘乙酸（MIA）诱导16只大鼠右膝关节炎。通过两次离心制备自体PRP，然后将其注射到8只大鼠的患关节炎膝关节中。该组被标记为治疗组（A），其余大鼠作为未治疗组（B）。在苏木精和伊红（H&E）染色的载玻片上对软骨细胞计数和未钙化软骨厚度进行形态学评估，结果发现治疗组A的软骨细胞计数高于未治疗组B，软骨高度也更高。使用独立t检验对治疗组（A）和未治疗组（B）进行组间比较。这些参数的P值具有统计学意义。结论：本研究得出结论，PRP对软骨细胞凋亡具有抑制作用，进而通过抑制基质丢失防止软骨高度降低。
