Hashimoto Hirotaka, Arai Kiyomi, Hayashi Shimmin, Okamoto Hiroyuki, Takahashi Jiro, Chikuda Makoto
Tsukuba Hashimoto Optical Clinic, 530 Furuku, Tsukuba, Ibaraki 305-0021, Japan; Department of Ophthalmology, Koshigaya Hospital, Dokkyo University School of Medicine, 2-1-50 Minamikoshigaya, Saitama 343-8555, Japan.
Department of Ophthalmology, Koshigaya Hospital, Dokkyo University School of Medicine, 2-1-50 Minamikoshigaya, Saitama 343-8555, Japan.
J Clin Biochem Nutr. 2016 Jul;59(1):10-5. doi: 10.3164/jcbn.15-137. Epub 2016 May 21.
We explored the effect of astaxanthin on vascular endothelial growth factor in the aqueous humor, by measuring vascular endothelial growth factor levels and oxidation-related parameters, including O2 (•-) scavenging activity, H2O2 level, and total hydroperoxide level in the aqueous humor, obtained from 35 patients before and after astaxanthin administration. We evaluated the relationship between vascular endothelial growth factor and the oxidation-related parameters as well as the patient's diabetic status, age, and sex. Vascular endothelial growth factor levels did not change significantly but O2 (•-) scavenging activity and total hydroperoxide level significantly (p<0.05) increased and decreased, respectively. Both pre- and post- astaxanthin intake, vascular endothelial growth factor and total hydroperoxide levels were positively correlated (Pearson: r = 0.42, p<0.05; r = 0.55, p<0.01, respectively). Analysis of vascular endothelial growth factor levels and O2 (•-) scavenging activities gave a negative correlation but only pre-astaxanthin intake (r = -0.37, p<0.05). Differences in levels pre- and post-astaxanthin only showed association between vascular endothelial growth factor and total hydroperoxide (r = 0.49, p<0.01) analyzed by multiple linear regression. Using multivariate analysis, pre-astaxanthin vascular endothelial growth factor level was associated with two factors of total hydroperoxide and O2 (•-) scavenging activity (r = 0.49, p<0.05), and post-astaxanthin vascular endothelial growth factor level with two factors of total hydroperoxide and sex (r = 0.60, p<0.01). Astaxanthin intake may have affected vascular endothelial growth factor level through its antioxidant effects by increasing O2 (•-) scavenging activity and suppressing peroxide production.
我们通过测量35例患者服用虾青素前后房水中血管内皮生长因子水平以及氧化相关参数，包括超氧阴离子（O2 (•-)）清除活性、过氧化氢（H2O2）水平和总氢过氧化物水平，探讨了虾青素对房水中血管内皮生长因子的影响。我们评估了血管内皮生长因子与氧化相关参数以及患者糖尿病状态、年龄和性别的关系。血管内皮生长因子水平无显著变化，但超氧阴离子（O2 (•-)）清除活性显著增加（p<0.05），总氢过氧化物水平显著降低（p<0.05）。在服用虾青素前后，血管内皮生长因子水平与总氢过氧化物水平均呈正相关（Pearson相关系数分别为：r = 0.42，p<0.05；r = 0.55，p<0.01）。血管内皮生长因子水平与超氧阴离子（O2 (•-)）清除活性分析呈负相关，但仅在服用虾青素前存在这种相关性（r = -0.37，p<0.05）。通过多元线性回归分析，虾青素服用前后水平差异仅显示血管内皮生长因子与总氢过氧化物之间存在关联（r = 0.49，p<0.01）。使用多变量分析，服用虾青素前血管内皮生长因子水平与总氢过氧化物和超氧阴离子（O2 (•-)）清除活性这两个因素相关（r = 0.49，p<0.05），服用虾青素后血管内皮生长因子水平与总氢过氧化物和性别这两个因素相关（r = 0.60，p<0.01）。虾青素的摄入可能通过增加超氧阴离子（O2 (•-)）清除活性和抑制过氧化物生成，发挥其抗氧化作用，从而影响血管内皮生长因子水平。
