Aprea Julieta, Lesche Mathias, Massalini Simone, Prenninger Silvia, Alexopoulou Dimitra, Dahl Andreas, Hiller Michael, Calegari Federico
DFG-Research Center and Cluster of Excellence for Regenerative Therapies; Dresden, Germany; Authors are equal contributing joint-first authors.
Deep Sequencing Group, Biotechnology Center; Dresden, Germany; Authors are equal contributing joint-first authors.
Neurogenesis (Austin). 2015 Apr 11;2(1):e995524. doi: 10.1080/23262133.2014.995524. eCollection 2015.
Long non-coding (lnc)RNAs play key roles in many biological processes. Elucidating the function of lncRNAs in cell type specification during organ development requires knowledge about their expression in individual progenitor types rather than in whole tissues. To achieve this during cortical development, we used a dual-reporter mouse line to isolate coexisting proliferating neural stem cells, differentiating neurogenic progenitors and newborn neurons and assessed the expression of lncRNAs by paired-end, high-throughput sequencing. We identified 379 genomic loci encoding novel lncRNAs and performed a comprehensive assessment of cell-specific expression patterns for all, annotated and novel, lncRNAs described to date. Our study provides a powerful new resource for studying these elusive transcripts during stem cell commitment and neurogenesis.
长链非编码（lnc）RNA在许多生物学过程中发挥关键作用。要阐明lncRNA在器官发育过程中细胞类型特化中的功能，需要了解它们在单个祖细胞类型中的表达情况，而非整个组织中的表达。为了在皮质发育过程中实现这一点，我们使用了一种双报告基因小鼠品系来分离共存的增殖神经干细胞、分化中的神经祖细胞和新生神经元，并通过双末端高通量测序评估lncRNA的表达。我们鉴定出379个编码新型lncRNA的基因组位点，并对迄今为止描述的所有已注释和新型lncRNA的细胞特异性表达模式进行了全面评估。我们的研究为在干细胞定向分化和神经发生过程中研究这些难以捉摸的转录本提供了一个强大的新资源。
