Song Yoo-Seok, Choi Yoorina, Lim Myung-Jin, Yu Mi-Kyung, Hong Chan-Ui, Lee Kwang-Won, Min Kyung-San
Department of Conservative Dentistry, School of Dentistry and Institute of Oral Bioscience, Chonbuk National University, Jeonju, Korea.
Department of Conservative Dentistry, Wonkwang University Dental Hospital, Iksan, Korea.
Restor Dent Endod. 2016 Aug;41(3):189-95. doi: 10.5395/rde.2016.41.3.189. Epub 2016 Jul 26.
A variety of root canal sealers were recently launched to the market. This study evaluated physicochemical properties, biocompatibility, and sealing ability of a newly launched resin-based sealer (Dia-Proseal, Diadent) compared to the existing root canal sealers (AHplus, Dentsply DeTrey and ADseal, Metabiomed).
The physicochemical properties of the tested sealers including pH, solubility, dimensional change, and radiopacity were evaluated. Biocompatibility was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. For microleakage test, single-rooted teeth were instrumented, and obturated with gutta-percha and one of the sealers (n = 10). After immersion in 1% methylene blue solution for 2 weeks, the specimens were split longitudinally. Then, the maximum length of staining was measured. Statistical analysis was performed by one-way analysis of variance followed by Tukey test (p = 0.05).
Dia-Proseal showed the highest pH value among the tested sealers (p < 0.05). ADseal showed higher dimensional change compared to AHplus and Dia-Proseal (p < 0.05). The solubility values of AHplus and Dia-Proseal were similar, whereas ADseal had the lowest solubility value (p < 0.05). The flow values of sealer in increasing order were AHplus, DiaProseal, and ADseal (p < 0.05). The radiopacity of AHplus was higher than those of ADseal and Dia-Proseal (p < 0.05). The cell viability of the tested materials was statistically similar throughout the experimental period. There were no significant differences in microleakage values among the tested samples.
The present study indicates that Dia-Proseal has acceptable physicochemical properties, biocompatibility, and sealing ability.
近期多种根管封闭剂投放市场。本研究评估了一种新推出的树脂基封闭剂（Dia-Proseal，迪阿登特）与现有根管封闭剂（AHplus，登士柏德瑞和ADseal，美塔生物医学）相比的物理化学性质、生物相容性及封闭能力。
评估受试封闭剂的物理化学性质，包括pH值、溶解度、尺寸变化及射线不透性。使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法测定生物相容性。对于微渗漏测试，对单根牙进行预备，并用牙胶和其中一种封闭剂进行充填（n = 10）。在1%亚甲蓝溶液中浸泡2周后，将标本纵向劈开。然后，测量染色的最大长度。采用单因素方差分析和Tukey检验进行统计学分析（p = 0.05）。
Dia-Proseal在受试封闭剂中显示出最高的pH值（p < 0.05）。与AHplus和Dia-Proseal相比，ADseal显示出更高的尺寸变化（p < 0.05）。AHplus和Dia-Proseal的溶解度值相似，而ADseal的溶解度值最低（p < 0.05）。封闭剂的流动值由低到高依次为AHplus、DiaProseal和ADseal（p < 0.05）。AHplus的射线不透性高于ADseal和Dia-Proseal（p < 0.05）。在整个实验期间，受试材料的细胞活力在统计学上相似。受试样品之间的微渗漏值无显著差异。
本研究表明，Dia-Proseal具有可接受的物理化学性质、生物相容性和封闭能力。
