从小花锦葵根皮中分离出的具有抗菌活性的β-谷甾醇的结构解析

Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora.

作者信息

Ododo Mesfin Medihin, Choudhury Manash Kumar, Dekebo Ahmed Hussen

机构信息

Chemistry Department, Aksum University, Aksum, Ethiopia.

Chemistry Department, Dilla University, P.O. Box 419, Dilla, Ethiopia.

出版信息

Springerplus. 2016 Jul 29;5(1):1210. doi: 10.1186/s40064-016-2894-x. eCollection 2016.

The powder of root bark of Malva parviflora (Malvaceae) was successively extracted with petroleum ether (b.p. 60-80 °C), chloroform and ethanol. The chloroform extract showed antibacterial activity against Staphylococcus aureus and Escherichia coli, whereas the ethanolic extract showed antibacterial activity against only S. aureus. The chloroform extract, after column chromatographic separation on silica gel using petroleum ether:chloroform (3:1) as eluent, furnished 98 mg of white crystalline compound. The yield of the compound is 0.316 % (w/w). The compound has a melting point of 134-136 °C and the Rf value 0.56 in benzene:chloroform:acetone (1:15:1) on silica gel TLC. The compound was characterized as β-sitosterol by physical properties, chemical test, spectral analysis (FTIR, NMR and MS) and comparing the data obtained from the literature.

小花锦葵（锦葵科）根皮粉末依次用石油醚（沸点60 - 80°C）、氯仿和乙醇提取。氯仿提取物对金黄色葡萄球菌和大肠杆菌显示出抗菌活性，而乙醇提取物仅对金黄色葡萄球菌显示出抗菌活性。氯仿提取物在硅胶柱上以石油醚：氯仿（3:1）为洗脱剂进行柱色谱分离后，得到98毫克白色结晶化合物。该化合物的产率为0.316%（重量/重量）。该化合物熔点为134 - 136°C，在硅胶薄层层析上于苯：氯仿：丙酮（1:15:1）中的比移值为0.56。通过物理性质、化学试验、光谱分析（傅里叶变换红外光谱、核磁共振和质谱）以及与文献数据比较，该化合物被鉴定为β-谷甾醇。