Dubert Javier, Romalde Jesús L, Spinard Edward J, Nelson David R, Gomez-Chiarri Marta, Barja Juan L
Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidade de Santiago de Compostela, Santiago de Compostela 15782, Spain.
Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA.
Int J Syst Evol Microbiol. 2016 Nov;66(11):4791-4796. doi: 10.1099/ijsem.0.001431. Epub 2016 Aug 18.
The Orientalis clade has a relevant significance for bivalve aquaculture since it includes the pathogens Vibrio bivalvicida, Vibrio tubiashii subsp. tubiashii and Vibrio tubiashii subsp. europaeus. However, the previous taxonomic description of the subspecies of V. tubiashii shows some incongruities that should be emended. In the genomic age, the comparison between genome assemblies is the key to clarify the taxonomic position of both subspecies. With this purpose, we have tested the ability of multilocus sequence analysis based on eight housekeeping gene sequences (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA and topA), different in silico genome-to-genome comparisons, chemotaxonomic features and phenotypic traits to reclassify the subspecies V. tubiashii subsp. europaeus within the Orientalis clade. This polyphasic approach clearly demonstrated that this subspecies is phylogenetically and phenotypically distinct from V. tubiashii and should be elevated to the rank of species as Vibrio europaeus sp. nov. This reclassification allows us to update the Orientalis clade (V. bivalvicida,V. brasiliensis, V. crosai, V. hepatarius, V. orientalis, V. sinaloensis, V. tubiashii and V. europaeus sp. nov.) and reconstruct a better phylogeny of the genus Vibrio. An emended description of V. tubiashii is provided. Finally, the proposed novel species is represented by emergent bivalve pathogens [type strain PP-638T (=CECT 8136T=DSM 27349T), PP2-843 and 07/118 T2] responsible for high mortalities in Spanish and French hatcheries.
东方分支对双壳贝类养殖具有重要意义，因为它包含双壳贝弧菌、塔氏弧菌 tubiashii 亚种和塔氏弧菌 europaeus 亚种等病原体。然而，先前对塔氏弧菌亚种的分类描述存在一些不一致之处，需要进行修订。在基因组时代，基因组组装之间的比较是明确这两个亚种分类地位的关键。为此，我们测试了基于八个管家基因序列（gapA、gyrB、ftsZ、mreB、pyrH、recA、rpoA 和 topA）的多位点序列分析能力、不同的计算机模拟基因组对基因组比较、化学分类特征和表型特征，以在东方分支内重新分类塔氏弧菌 europaeus 亚种。这种多相方法清楚地表明，该亚种在系统发育和表型上与塔氏弧菌不同，应提升为新种欧洲弧菌（Vibrio europaeus sp. nov.）的等级。这一重新分类使我们能够更新东方分支（双壳贝弧菌、巴西弧菌、克罗弧菌、肝弧菌、东方弧菌、锡那罗亚弧菌、塔氏弧菌和新种欧洲弧菌）并重建更好的弧菌属系统发育。提供了对塔氏弧菌的修订描述。最后，所提议的新物种由导致西班牙和法国孵化场高死亡率的新兴双壳贝类病原体（模式菌株 PP - 638T = CECT 8136T = DSM 27349T、PP2 - 843 和 07/118 T2）代表。
