Vibrio fujianensis sp. nov., isolated from aquaculture water.

A Gram-stain-negative, facultatively anaerobic strain, designated FJ201301, was isolated from aquaculture water collected from Fujian province, China. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain FJ201301 belonged to the genus Vibrio, formed a distinct cluster with Vibriocincinnatiensis ATCC 35912 and shared the highest similarity with Vibriosalilacus CGMCC 1.12427. A 15 bp insertion found in the 16S rRNA gene was a significant marker that distinguished strain FJ201301 from several phylogenetic neighbours (e.g. V. cincinnatiensis). Multilocus sequence analysis of eight genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA and topA; concatenated 4135 bp sequence) showed that, forming a long and independent phylogenetic branch, strain FJ201301 clustered with V. cincinnatiensis ATCC 35912, Vibrioinjenensis KCTC 32233 and Vibriometschnikovii CIP 69.14 clearly separated from V. salilacus CGMCC 1.12427. Furthermore, the highest in silico DNA-DNA hybridization and average nucleotide identity values between strain FJ201301 and the closest related species were 26.3 and 83.1 % with V. cincinnatiensis ATCC 35912, less than the proposed cutoff levels for species delineation, i.e. 70 and 95 %, respectively. Biochemical, sequence and genomic analysis suggested the designation of strain FJ201301 representing a novel species of the genus Vibrio, for which the name Vibrio fujianensis sp. nov. is proposed. The type strain is FJ201301 (=DSM 104687=CGMCC 1.16099).