Fang Yujie, Chen Aiping, Dai Hang, Huang Ying, Kan Biao, Wang Duochun
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, PR China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, PR China.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1146-1152. doi: 10.1099/ijsem.0.002642. Epub 2018 Feb 13.
A Gram-stain-negative, facultatively anaerobic strain, designated FJ201301, was isolated from aquaculture water collected from Fujian province, China. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain FJ201301 belonged to the genus Vibrio, formed a distinct cluster with Vibriocincinnatiensis ATCC 35912 and shared the highest similarity with Vibriosalilacus CGMCC 1.12427. A 15 bp insertion found in the 16S rRNA gene was a significant marker that distinguished strain FJ201301 from several phylogenetic neighbours (e.g. V. cincinnatiensis). Multilocus sequence analysis of eight genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA and topA; concatenated 4135 bp sequence) showed that, forming a long and independent phylogenetic branch, strain FJ201301 clustered with V. cincinnatiensis ATCC 35912, Vibrioinjenensis KCTC 32233 and Vibriometschnikovii CIP 69.14 clearly separated from V. salilacus CGMCC 1.12427. Furthermore, the highest in silico DNA-DNA hybridization and average nucleotide identity values between strain FJ201301 and the closest related species were 26.3 and 83.1 % with V. cincinnatiensis ATCC 35912, less than the proposed cutoff levels for species delineation, i.e. 70 and 95 %, respectively. Biochemical, sequence and genomic analysis suggested the designation of strain FJ201301 representing a novel species of the genus Vibrio, for which the name Vibrio fujianensis sp. nov. is proposed. The type strain is FJ201301 (=DSM 104687=CGMCC 1.16099).
从中国福建省采集的水产养殖用水中分离出一株革兰氏阴性兼性厌氧菌株，命名为FJ201301。16S rRNA基因序列的系统发育分析表明，菌株FJ201301属于弧菌属，与辛辛那提弧菌ATCC 35912形成一个独特的聚类，与盐湖弧菌CGMCC 1.12427的相似性最高。在16S rRNA基因中发现的一个15bp的插入是一个重要的标记，可将菌株FJ201301与其几个系统发育近缘种（如辛辛那提弧菌）区分开来。对八个基因（ftsZ、gapA、gyrB、mreB、pyrH、recA、rpoA和topA；串联的4135bp序列）进行多位点序列分析表明，菌株FJ201301形成一个长且独立的系统发育分支，与辛辛那提弧菌ATCC 35912、仁川弧菌KCTC 32233和梅契尼科夫弧菌CIP 69.14聚类，明显与盐湖弧菌CGMCC 1.12427分开。此外，菌株FJ201301与最密切相关物种之间的最高计算机模拟DNA-DNA杂交和平均核苷酸同一性值分别为26.3%和83.1%（与辛辛那提弧菌ATCC 35912相比），低于物种划分的建议临界值，即分别为70%和95%。生化、序列和基因组分析表明，菌株FJ201301代表弧菌属的一个新物种，为此提出了福建弧菌新种（Vibrio fujianensis sp. nov.）的名称。模式菌株为FJ201301（=DSM 104687=CGMCC 1.16099）。
