Rota P A, Rocha E P, Harmon M W, Hinshaw V S, Sheerar M G, Kawaoka Y, Cox N J, Smith T F
Division of Viral Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1989 Jun;27(6):1413-6. doi: 10.1128/jcm.27.6.1413-1416.1989.
A swine influenza virus-like type A (H1N1) virus, designated A/Wisconsin/3523/88, was isolated in September 1988 from a Wisconsin woman who had died with primary viral pneumonia. Antigenic analyses with hemagglutinin-specific monoclonal antibodies and postinfection ferret serum indicated that the hemagglutinin of A/Wisconsin/3523/88 was antigenically closely related to viruses currently circulating in swine. Genetic analysis of the A/Wisconsin/3523/88 virus by RNA fingerprinting and partial RNA sequence analysis of seven of the eight segments indicated that the genome of the human isolate was similar to that of enzootic swine viruses. These laboratory data supported the epidemiologic findings that this human infection occurred by transmission of an enzootic swine influenza virus and that the virus showed no major genetic changes potentially related to increased pathogenesis.
1988年9月，从一名因原发性病毒性肺炎死亡的威斯康星州妇女身上分离出一种类似甲型流感病毒的A型（H1N1）病毒，命名为A/威斯康星/3523/88。用血凝素特异性单克隆抗体和感染后雪貂血清进行的抗原分析表明，A/威斯康星/3523/88的血凝素在抗原性上与目前在猪中传播的病毒密切相关。通过RNA指纹图谱和八个基因节段中七个的部分RNA序列分析对A/威斯康星/3523/88病毒进行的基因分析表明，该人类分离株的基因组与地方性猪病毒的基因组相似。这些实验室数据支持了流行病学研究结果，即这种人类感染是由一种地方性猪流感病毒传播引起的，并且该病毒没有显示出可能与发病机制增加相关的重大基因变化。
