Rekik M R, Arora D J, Dea S
Institut Armand-Frappier, Laval, Québec, Canada.
J Clin Microbiol. 1994 Feb;32(2):515-8. doi: 10.1128/jcm.32.2.515-518.1994.
A new antigenic variant of H1N1 swine influenza virus A (Sw/QC/5393/91 [QC/91]) has been found to be associated with porcine proliferative and necrotizing pneumonia. Analysis of its genomic RNA by T1 oligonucleotide mapping revealed that considerable genomic divergence exists between QC/91 and the swine influenza viruses currently circulating in North American swine herds. Analysis of the nucleotide sequence of the HA1 region of the hemagglutinin RNA of QC/91, in comparison with those of most common H1N1 human and swine influenza A viruses, showed the presence of multiple point mutations. Two amino acid substitutions appeared to be located in antigenic sites Sb and Ca. This correlates with antigenic variations demonstrated between A/NJ/8/76, A/Sw/WI/49/76, and Québec isolate A/Sw/QC/5393/91 of swine influenza virus A. Another mutation was responsible for the loss of a glycosylation site, which may have also affected the antigenicity. The other mutations seem to have been accumulated progressively over time. This significant constancy in the fixation of mutations with time suggests that genetic diversity of these viruses may best be interpreted as the result of drifts in the population of circulating swine influenza viruses in Québec.
已发现一种新的甲型H1N1猪流感病毒抗原变异株（Sw/QC/5393/91 [QC/91]）与猪增生性坏死性肺炎有关。通过T1寡核苷酸图谱分析其基因组RNA发现，QC/91与目前在北美猪群中传播的猪流感病毒之间存在相当大的基因组差异。将QC/91血凝素RNA的HA1区域核苷酸序列与最常见的甲型H1N1人源和猪源流感病毒的序列进行比较，发现存在多个点突变。两个氨基酸取代似乎位于抗原位点Sb和Ca。这与甲型猪流感病毒A/NJ/8/76、A/Sw/WI/49/76和魁北克分离株A/Sw/QC/5393/91之间显示的抗原变异相关。另一个突变导致一个糖基化位点缺失，这也可能影响了抗原性。其他突变似乎是随着时间逐渐积累的。这种突变随时间固定的显著稳定性表明，这些病毒的遗传多样性最好解释为魁北克流行猪流感病毒群体中漂移的结果。
