Anghebem-Oliveira Mauren Isfer, Webber Susan, Alberton Dayane, de Souza Emanuel Maltempi, Klassen Giseli, Picheth Geraldo, Rego Fabiane Gomes de Moraes
Department of Clinical Analysis, Federal University of Parana, Curitiba, Brazil.
Health and Biosciences School, Pontifical Catholic University of Parana, Curitiba, Brazil.
J Clin Lab Anal. 2017 Mar;31(2). doi: 10.1002/jcla.22035. Epub 2016 Aug 24.
The glucokinase regulatory protein (GCKR) regulates the activity of the glucokinase (GCK), which plays a key role in glucose homeostasis. Genetic variants in GCK have been associated with diabetes and gestational diabetes (GDM). Due to the relationship between GCKRP and GCK, polymorphisms in GCKR are also candidates for genetic association with GDM. The aim of this study was to evaluate the association between the GCKR rs780094 polymorphism and GDM in a Brazilian population.
252 unrelated Euro-Brazilian pregnant women were classified as control (healthy pregnant women, n = 125) and GDM (pregnant women with GDM, n = 127) age-matched groups. Clinical and anthropometric data were obtained from all subjects. The GCKR rs780094 polymorphism was genotyped using fluorescent probes (TaqMan , code C_2862873_10).
Both groups were in Hardy-Weinberg equilibrium. The GCKR rs780094 polymorphism was associated with GDM in codominant and dominant models (P = 0.022 and P = 0.010, respectively). The minor allele (T) frequency for the control group in the study was 38.4% (95% CI: 32-44%), similar to frequencies reported for other Caucasian populations.
Carriers of the C allele of rs780094 were 1.41 (odds ratio, 95% CI, 0.97-2.03) times more likely to develop GDM.
葡萄糖激酶调节蛋白（GCKR）调节葡萄糖激酶（GCK）的活性，而葡萄糖激酶在葡萄糖稳态中起关键作用。GCK的基因变异与糖尿病和妊娠期糖尿病（GDM）有关。由于GCKRP与GCK之间的关系，GCKR中的多态性也是与GDM发生遗传关联的候选因素。本研究旨在评估巴西人群中GCKR rs780094多态性与GDM之间的关联。
将252名无亲缘关系的欧洲裔巴西孕妇分为年龄匹配的对照组（健康孕妇，n = 125）和GDM组（患有GDM的孕妇，n = 127）。从所有受试者中获取临床和人体测量数据。使用荧光探针（TaqMan，编号C_2862873_10）对GCKR rs780094多态性进行基因分型。
两组均处于哈迪-温伯格平衡。GCKR rs780多态性在共显性和显性模型中与GDM相关（分别为P = 0.022和P = 0.010）。本研究中对照组的次要等位基因（T）频率为38.4%（95%CI：32 - 44%），与其他高加索人群报道的频率相似。
rs780094的C等位基因携带者患GDM的可能性是1.41倍（优势比，95%CI，0.97 - 2.03）。
