Drago Ilaria, Davis Ronald L
Department of Neuroscience, The Scripps Research Institute Florida, Jupiter, FL 33458, USA.
Department of Neuroscience, The Scripps Research Institute Florida, Jupiter, FL 33458, USA.
Cell Rep. 2016 Sep 6;16(10):2763-2776. doi: 10.1016/j.celrep.2016.08.017. Epub 2016 Aug 25.
The uptake of cytoplasmic calcium into mitochondria is critical for a variety of physiological processes, including calcium buffering, metabolism, and cell survival. Here, we demonstrate that inhibiting the mitochondrial calcium uniporter in the Drosophila mushroom body neurons (MBn)-a brain region critical for olfactory memory formation-causes memory impairment without altering the capacity to learn. Inhibiting uniporter activity only during pupation impaired adult memory, whereas the same inhibition during adulthood was without effect. The behavioral impairment was associated with structural defects in MBn, including a decrease in synaptic vesicles and an increased length in the axons of the αβ MBn. Our results reveal an in vivo developmental role for the mitochondrial uniporter complex in establishing the necessary structural and functional neuronal substrates for normal memory formation in the adult organism.
细胞质钙摄入线粒体对于多种生理过程至关重要，包括钙缓冲、新陈代谢和细胞存活。在此，我们证明，在果蝇蘑菇体神经元（MBn）——对嗅觉记忆形成至关重要的脑区——中抑制线粒体钙单向转运体，会导致记忆受损，而不会改变学习能力。仅在化蛹期间抑制单向转运体活性会损害成年果蝇的记忆，而在成年期进行同样的抑制则没有效果。行为障碍与MBn的结构缺陷有关，包括突触小泡减少和αβ MBn轴突长度增加。我们的结果揭示了线粒体单向转运体复合物在成年生物体中为正常记忆形成建立必要的结构和功能神经元底物方面的体内发育作用。
