Nordbring-Hertz B, Friman E, Veenhuis M
Department of Microbial Ecology, University of Lund, Sweden.
Antonie Van Leeuwenhoek. 1989 Mar;55(3):237-44. doi: 10.1007/BF00393852.
Hyphal fusion during initial stages of trap formation by Arthrobotrys oligospora was studied by video-enhanced contrast and electron microscopy. Trap initials grew perpendicularly to the parent hypha, then curved around and anastomosed with a peg that developed on the hypha. Trap initials usually developed 40-140 microns apart while the anastomosis occurred 20-25 microns from the initial. Vigorous cytoplasmic movements in trap initials and developed traps corresponded to intense staining with fluorescein diacetate (FDA) of these cells. In addition, bundles of microfilaments were seen in developing loops of traps. On fusion organelle migration took place from the tip cell of the trap into the peg. Later on a septum was formed at the site of fusion.
利用视频增强对比度和电子显微镜研究了少孢节丛孢菌在捕虫器形成初始阶段的菌丝融合情况。捕虫器原基垂直于亲本菌丝生长，然后弯曲并与菌丝上形成的一个短柱体吻合。捕虫器原基通常相隔40 - 140微米发育，而吻合发生在距原基20 - 25微米处。捕虫器原基和发育成熟的捕虫器中有活跃的细胞质运动，这与这些细胞被二乙酸荧光素（FDA）强烈染色相对应。此外，在捕虫器发育的环中可见微丝束。在融合时，细胞器从捕虫器的顶端细胞迁移到短柱体中。随后在融合部位形成隔膜。
