Jeong Da-Hyun, Kim Koth-Bong-Woo-Ri, Kim Min-Ji, Kang Bo-Kyeong, Ahn Dong-Hyun
Department of Food Science and Technology, Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea.
Institute of Fisheries Sciences, Pukyong National University, 474, Ilgwang-ro, Ilgwang-myeon, Gijang-gun, Busan 619-911, Republic of Korea.
Int Immunopharmacol. 2016 Nov;40:50-56. doi: 10.1016/j.intimp.2016.07.005. Epub 2016 Aug 28.
The effect of tuna eyeball oil (TEO) on lipopolysaccharide (LPS)-induced inflammation in macrophage cells was investigated. TEO had no cytotoxicity in cell viability as compared to the control in LPS induced RAW 264.7 cells. TEO reduced the levels of NO and pro-inflammatory cytokines by up to 50% in a dose-dependent manner. The expression of NF-κB and MAPKs as well as iNOS and COX-2 proteins was reduced by TEO, which suggests that its anti-inflammatory activity is related to the suppression of the NF-κB and MAPK signaling pathways. The rate of formation of ear edema was reduced compared to that in the control at the highest dose tested. In an acute toxicity test, no mice were killed by TEO doses of up to 5000mg/kg body weight during the two week observation period. These results suggested that TEO may have a significant effect on inflammatory factors and be a potential anti-inflammatory therapeutic.
研究了金枪鱼眼球油（TEO）对巨噬细胞中脂多糖（LPS）诱导的炎症的影响。与LPS诱导的RAW 264.7细胞中的对照相比，TEO对细胞活力没有细胞毒性。TEO以剂量依赖性方式将NO和促炎细胞因子水平降低多达50%。TEO降低了NF-κB和MAPKs以及iNOS和COX-2蛋白的表达，这表明其抗炎活性与抑制NF-κB和MAPK信号通路有关。在测试的最高剂量下，与对照组相比，耳部水肿的形成率降低。在急性毒性试验中，在两周观察期内，体重高达5000mg/kg的TEO剂量未导致小鼠死亡。这些结果表明，TEO可能对炎症因子有显著影响，是一种潜在的抗炎治疗剂。
