香青兰水醇提取物通过调节葡萄糖转运蛋白 2 和 4 的表达和抑制氧化应激对链脲佐菌素诱导的糖尿病的保护作用。

CONTEXT

Oxidative stress plays an important role in development of diabetes mellitus. Satureja bachtiarica Bunge (Lamiaceae) is a rich source of bioactive compounds with antioxidant properties.

OBJECTIVE

This study investigates the antidiabetic effect of hydroalcoholic extract of aerial parts of S. bachtiarica.

METHODS AND MATERIALS

Male Wistar rats were randomly divided into six groups (n = 8) including control (normal saline), diabetic [Streptozotocin (STZ)], intervention (STZ plus hydroalcoholic extract of S. bachtiarica at doses of 75, 150 and 250 mg/kg/d) and positive control (STZ plus captopril 50 mg/kg/d) groups. A single intraperitoneal (IP) injection of STZ (60 mg/kg) was used to induce diabetes and IP therapy with drugs was performed for four weeks.

RESULTS

In diabetic rats, serum total antioxidant capacity (TAC) decreased significantly, but glucose, alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (GGT) and malondialdehyde (MDA) increased significantly as compared to the control (p < 0.05). Treatment with extract (250 mg/kg) caused a significant decline in serum glucose, GGT, ALT, AST and MDA as well as a significant increase in serum TAC (p < 0.05). During the intervention period, diabetic rats showed significant weight loss, but extract (250 mg/kg) treated rats did not show any weight loss. Extract (250 mg/kg) up-regulated GLUT2 expression and down-regulated GLUT4 expression in the liver (p < 0.05). S. bachtiarica extract at all dosage levels prevented STZ-induced histological damage of liver, kidney and pancreas.

DISCUSSION AND CONCLUSIONS

S. bachtiarica extract exhibits antidiabetic effects through modulation of oxidative stress and expression of GLUT2 and GLUT4.

背景

氧化应激在糖尿病的发生发展中起着重要作用。香薷（Bunge）（唇形科）是生物活性化合物的丰富来源，具有抗氧化特性。

目的

本研究探讨了香薷地上部分水醇提取物的抗糖尿病作用。

方法和材料

雄性 Wistar 大鼠随机分为六组（n=8），包括对照组（生理盐水）、糖尿病组[链脲佐菌素（STZ）]、干预组（STZ 加香薷水醇提取物，剂量分别为 75、150 和 250mg/kg/d）和阳性对照组（STZ 加卡托普利 50mg/kg/d）。通过单次腹腔（IP）注射 STZ（60mg/kg）诱导糖尿病，并用药物进行 IP 治疗 4 周。

结果

与对照组相比，糖尿病大鼠血清总抗氧化能力（TAC）显著降低，而血糖、碱性磷酸酶（ALP）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、γ-谷氨酰转移酶（GGT）和丙二醛（MDA）显著升高（p<0.05）。用提取物（250mg/kg）治疗可显著降低血清葡萄糖、GGT、ALT、AST 和 MDA，同时显著增加血清 TAC（p<0.05）。在干预期间，糖尿病大鼠体重明显减轻，但用提取物（250mg/kg）治疗的大鼠体重没有减轻。提取物（250mg/kg）上调肝脏 GLUT2 表达，下调 GLUT4 表达（p<0.05）。香薷提取物在所有剂量水平均可预防 STZ 诱导的肝、肾和胰腺组织损伤。