Wulstein Devynn M, Regan Kathryn E, Robertson-Anderson Rae M, McGorty Ryan
Opt Express. 2016 Sep 5;24(18):20881-94. doi: 10.1364/OE.24.020881.
Using light-sheet microscopy combined with digital Fourier methods we probe the dynamics of colloidal samples and DNA molecules. This combination, referred to as selective-plane illumination differential dynamic microscopy (SPIDDM), has the benefit of optical sectioning to study, with minimal photobleaching, thick samples allowing us to measure the diffusivity of colloidal particles at high volume fractions. Further, SPIDDM exploits the inherent spatially-varying thickness of Gaussian light-sheets. Where the excitation sheet is most focused, we capture high spatial frequency dynamics as the signal-to-background is high. In thicker regions, we capture the slower dynamics as diffusion out of the sheet takes longer.
我们使用光片显微镜结合数字傅里叶方法来探测胶体样品和DNA分子的动力学。这种结合，即选择性平面照明显微差分动态显微镜（SPIDDM），具有光学切片的优势，能够以最小的光漂白来研究厚样品，使我们能够在高体积分数下测量胶体颗粒的扩散系数。此外，SPIDDM利用了高斯光片固有的空间变化厚度。在激发光片聚焦最强的地方，由于信号背景比高，我们捕获高空间频率的动力学。在较厚的区域，由于扩散出光片需要更长时间，我们捕获较慢的动力学。
