Asahara Hiroshi
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA.; Department of Systems BioMedicine, Tokyo Medical and Dental University, Tokyo, Japan.; The Core Research for the Evolutionary Science and Technology from Japan Agency for Medical Research and Development, Tokyo, Japan.
J Bone Metab. 2016 Aug;23(3):121-7. doi: 10.11005/jbm.2016.23.3.121. Epub 2016 Aug 31.
MicroRNAs (miRNAs), which are small (~21 nucleotides) non-coding RNAs, are important players in endochondral ossification, articular cartilage homeostasis, and arthritis pathogenesis. Comprehensive and genetic analyses of cartilage-specific or cartilage-related miRNAs have provided new information on cartilage development, homeostasis, and related diseases. State-of-the-art combinatorial approaches, including transcription-activator like effector nuclease (TALEN)/clustered regularly interspaced short palindromic repeats (CRISPR) technique for targeting miRNAs and high-throughput sequencing of RNA isolated by cross-linking immunoprecipitation for identifying target messenger RNAs, should be used to determine complex miRNA networks and miRNA-dependent cartilage regulation. Use of advanced drug delivery systems involving cartilage-specific miRNAs will accelerate the application of these new findings in arthritis therapy.
微小RNA（miRNA）是一类小的（约21个核苷酸）非编码RNA，是软骨内骨化、关节软骨稳态和关节炎发病机制中的重要参与者。对软骨特异性或软骨相关miRNA的全面和遗传学分析为软骨发育、稳态及相关疾病提供了新信息。应采用包括用于靶向miRNA的转录激活因子样效应物核酸酶（TALEN）/成簇规律间隔短回文重复序列（CRISPR）技术以及用于鉴定靶信使RNA的交联免疫沉淀法分离RNA的高通量测序等先进的组合方法，以确定复杂的miRNA网络和miRNA依赖性软骨调节。涉及软骨特异性miRNA的先进药物递送系统的应用将加速这些新发现在关节炎治疗中的应用。
