Li Hui, Ji Qiuxia, Chen Ximin, Sun Yan, Xu Quanchen, Deng Panpan, Hu Fang, Yang Jianjun
Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266001, China.
School of Stomatology, Qingdao University, Qingdao, Shandong, 266001, China.
J Biomed Mater Res A. 2017 Jan;105(1):265-273. doi: 10.1002/jbm.a.35900. Epub 2016 Oct 25.
Regeneration of alveolar bone is among the main purposes of periodontal treatment. However, it is a challenge for both dentists and patients. An injectable chitosan-based thermosensitive hydrogel scaffold (CS/CSn-GP) was successfully designed and prepared with the incorporation of bone morphogenetic protein-2 plasmid DNA (pDNA-BMP2)-loaded chitosan nanoparticles (CS/CSn(pDNA-BMP2)-GP) in this study. The aim of this study was to evaluate the regenerative effect of this scaffold on the endogenous repair of alveolar bone. Thermosensitive properties, SEM scans and in vivo inflammatory reactions and degradation were evaluated. The CS/CSn-GP solution was initially injected into the muscle pouches of rats, where it changed into a gel, resulting in non-specific inflammation. It effectively enhanced new bone formation in rat calvarial defects and enhanced bony defect healing in beagle dogs. All results indicated that the CS/CSn(pDNA-BMP2)-GP complex system is a potential candidate as a targeted, multi-effect scaffold for the endogenous repair of alveolar bone. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 265-273, 2017.
牙槽骨再生是牙周治疗的主要目的之一。然而，这对牙医和患者来说都是一项挑战。在本研究中，通过将负载骨形态发生蛋白-2质粒DNA（pDNA-BMP2）的壳聚糖纳米颗粒（CS/CSn(pDNA-BMP2)-GP）掺入其中，成功设计并制备了一种基于壳聚糖的可注射热敏水凝胶支架（CS/CSn-GP）。本研究的目的是评估该支架对牙槽骨内源性修复的再生效果。对热敏性能、扫描电子显微镜（SEM）扫描以及体内炎症反应和降解情况进行了评估。CS/CSn-GP溶液最初被注射到大鼠的肌肉袋中，在那里它变成凝胶，导致非特异性炎症。它有效促进了大鼠颅骨缺损处的新骨形成，并促进了比格犬骨缺损的愈合。所有结果表明，CS/CSn(pDNA-BMP2)-GP复合系统作为一种用于牙槽骨内源性修复的靶向、多效支架具有潜在的应用前景。©2016威利期刊公司。《生物医学材料研究杂志》A部分：第105A卷：265 - 273页，2017年。
