Devi Palanisamy Bruntha, Kavitake Digambar, Shetty Prathapkumar Halady
Department of Microbial Biotechnology, Bharathiar University, Coimbatore-641046, India.
Department of Food Science and Technology, Pondicherry University, Pondicherry 605014, India.
Int J Biol Macromol. 2016 Dec;93(Pt A):822-828. doi: 10.1016/j.ijbiomac.2016.09.054. Epub 2016 Sep 17.
Physico-chemical properties of a galactan exopolysaccharide (EPS) produced by Weissella confusa KR780676 isolated from an idli batter were studied. In our previous paper, we had reported the isolation and characterization of a linear galactan (containing α-(1→6)-linked galactose units) isolated from an acidic fermented food. In this manuscript, we are reporting the physico-chemical properties viz., Colour evaluation (L* 81.79, a* 3.15, b* 15.04), flow properties (compressibility index 38.46%), syneresis, light transmittance (50.7% for 5days), pasting properties (temperature 64.6°C), texture (hardness 9.876N) and particle size (549.81dnm) for the galactan were carried out. This galactan also possessed high oil absorption capacity (5.09mL/g) with good emulsifying activity (69.4%) and emulsion stability up to 15days. Flocculating activity of 65.3-81.7% was observed with 0.2mg/mL EPS in a wide range of pH (2.0-12.0). These functional properties will make the galactan EPS a good candidate for use as thickening and gelling agent and emulsifier to form long-term emulsions in food, pharmaceutical and cosmetic products, as well as for bio-treatment of wastewater and hydrocarbon-polluted environments.
对从一种印度酸豆面糊中分离出的困惑魏斯氏菌KR780676产生的一种半乳聚糖胞外多糖(EPS)的物理化学性质进行了研究。在我们之前的论文中,我们报道了从一种酸性发酵食品中分离出的一种线性半乳聚糖(含有α-(1→6)连接的半乳糖单元)及其特性。在本手稿中,我们报告了该半乳聚糖的物理化学性质,即颜色评价(L* 81.79,a* 3.15,b* 15.04)、流动性质(压缩性指数38.46%)、脱水收缩、透光率(5天为50.7%)、糊化性质(温度64.6°C)、质地(硬度9.876N)和粒径(549.81dnm)。这种半乳聚糖还具有高吸油能力(5.09mL/g),具有良好的乳化活性(69.4%),乳液稳定性可达15天。在广泛的pH范围(2.0 - 12.0)内,0.2mg/mL EPS的絮凝活性为65.3 - 81.7%。这些功能特性将使半乳聚糖EPS成为一种很好的候选物质,可用于食品、制药和化妆品中作为增稠剂、胶凝剂和乳化剂以形成长期乳液,以及用于废水和烃污染环境的生物处理。