Kavitake Digambar, Balyan Sangeeta, Devi Palanisamy Bruntha, Shetty Prathapkumar Halady
Department of Food Science and Technology, Pondicherry University, Pondicherry, 605014 India.
J Food Sci Technol. 2020 Apr;57(4):1579-1585. doi: 10.1007/s13197-020-04262-3. Epub 2020 Jan 24.
Galactan exopolysaccharide (EPS) was extracted from KR780676 isolated from batter. The present study reports the effect of galactan EPS concentration, pH, ionic strength, temperature, salinity, monovalent salts on the emulsion formed with vegetable oils (virgin coconut oil, groundnut oil, olive oil and mustard oil). Emulsion determination test revealed the oil-in-water type of emulsion. Microscopic observation showed the phase of oil droplet distribution of emulsions. Groundnut and olive oil showed significant emulsifying activity (50-60%) and stability (90%) irrespective of the EPS concentration. One percent of EPS also showed good emulsifying activity (50-70%) and stability (100%) with groundnut and olive oil in wide range of temperature (- 20, 4, 30, 40, 50 and 60 °C), pH (3-8), salinity (1, 5, 10, 20 and 30% of NaCl) and monovalent salt (1, 5, 10, 20 and 30% of KCl). Galactan could be used as a prominent emulsifier for applications in the food industry as it possesses significant activity with most of the influential factors in wide range and also obtained from lactic acid bacteria that are regarded as Generally Regarded as Safe (GRAS).
从面糊中分离出的KR780676中提取了半乳聚糖胞外多糖(EPS)。本研究报告了半乳聚糖EPS浓度、pH值、离子强度、温度、盐度、单价盐对与植物油(初榨椰子油、花生油、橄榄油和芥菜油)形成的乳液的影响。乳液测定试验表明形成的是水包油型乳液。显微镜观察显示了乳液中油滴的分布相。无论EPS浓度如何,花生油和橄榄油都表现出显著的乳化活性(50-60%)和稳定性(90%)。1%的EPS在较宽的温度范围(-20、4、30、40、50和60°C)、pH值(3-8)、盐度(1、5、10、20和30%的NaCl)和单价盐(1、5、10、20和30%的KCl)条件下,与花生油和橄榄油也表现出良好的乳化活性(50-70%)和稳定性(100%)。半乳聚糖可作为一种重要的乳化剂用于食品工业,因为它在较宽范围内对大多数影响因素都具有显著活性,并且是从被视为一般认为安全(GRAS)的乳酸菌中获得的。