Jiang Pengfei, Zhang Yixian, Zhu Chaonan, Zhang Wenjing, Mao Zhengwei, Gao Changyou
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Acta Biomater. 2016 Dec;46:141-150. doi: 10.1016/j.actbio.2016.09.020. Epub 2016 Sep 16.
UNLABELLED: Differentiation of stem cells is influenced by many factors, yet uptake of the magnetic particles with or without magnetic field is rarely tackled. In this study, iron oxide nanoparticles-loaded bovine serum albumin (BSA) (FeO/BSA) particles were prepared, which showed a spherical morphology with a diameter below 200 nm, negatively charged surface, and tunable magnetic property. The particles could be internalized into bone marrow mesenchymal stem cells (MSCs), and their release from the cells was significantly retarded under external magnetic field, resulting in almost twice intracellular amount of the particles within 21 d compared to that of the magnetic field free control. Uptake of the FeO/BSA particles enhanced significantly the osteogenic differentiation of MSCs under a static magnetic field, as evidenced by elevated alkaline phosphatase (ALP) activity, calcium deposition, and expressions of collagen type I and osteocalcin at both mRNA and protein levels. Therefore, uptake of the FeO/BSA particles brings significant influence on the differentiation of MSCs under magnetic field, and thereby should be paid great attention for practical applications. STATEMENT OF SIGNIFICANCE: Differentiation of stem cells is influenced by many factors, yet uptake of the magnetic particles with or without magnetic field is rarely tackled. In this study, iron oxide nanoparticles-loaded bovine serum albumin (BSA) (FeO/BSA) particles with a diameter below 200nm, negatively charged surface, tunable FeO content and subsequently adjustable magnetic property were prepared. The particles could be internalized into bone marrow mesenchymal stem cells (MSCs), and their release from the cells was significantly retarded under external magnetic field. Uptake of the FeO/BSA particles enhanced significantly the osteogenic differentiation of MSCs under a constant static magnetic field, while the magnetic particles and external magnetic field alone do not influence significantly the osteogenic differentiation potential of MSCs regardless of the uptake amount. The results demonstrate a potential magnetic manipulation method for stem cell differentiation, and also convey the significance of careful evaluation of the safety issue of magnetic particles in real an application situation.
未标注：干细胞的分化受多种因素影响，但很少有人研究有无磁场情况下磁性颗粒的摄取情况。在本研究中，制备了负载氧化铁纳米颗粒的牛血清白蛋白（FeO/BSA）颗粒，其呈球形，直径小于200nm，表面带负电荷，且具有可调磁性。这些颗粒能够被内化到骨髓间充质干细胞（MSC）中，在外部磁场作用下，它们从细胞中的释放显著延迟，与无磁场对照相比，在21天内细胞内颗粒量几乎增加了一倍。在静磁场下，FeO/BSA颗粒的摄取显著增强了MSC的成骨分化，碱性磷酸酶（ALP）活性升高、钙沉积以及I型胶原蛋白和骨钙素在mRNA和蛋白质水平的表达均证明了这一点。因此，FeO/BSA颗粒的摄取在磁场作用下对MSC的分化有显著影响，在实际应用中应予以高度重视。 意义声明：干细胞的分化受多种因素影响，但很少有人研究有无磁场情况下磁性颗粒的摄取情况。在本研究中，制备了直径小于200nm、表面带负电荷、FeO含量可调且磁性随后可调节的负载氧化铁纳米颗粒的牛血清白蛋白（FeO/BSA）颗粒。这些颗粒能够被内化到骨髓间充质干细胞（MSC）中，在外部磁场作用下，它们从细胞中的释放显著延迟。在恒定静磁场下，FeO/BSA颗粒的摄取显著增强了MSC的成骨分化，而单独的磁性颗粒和外部磁场无论摄取量如何都不会显著影响MSC的成骨分化潜能。结果证明了一种用于干细胞分化的潜在磁操纵方法，同时也传达了在实际应用中仔细评估磁性颗粒安全性问题的重要性。
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