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壳聚糖/海藻酸钠/CuO-NPs 和壳聚糖/海藻酸钠/FeO-NPs 支架上绵羊胎儿骨髓间充质干细胞向成骨细胞分化的比较。

Comparison of the differentiation of ovine fetal bone-marrow mesenchymal stem cells towards osteocytes on chitosan/alginate/CuO-NPs and chitosan/alginate/FeO-NPs scaffolds.

机构信息

Department of Animal Sciences, College of Agriculture and Natural Resources, Razi University, Kermanshah, 67144-14971, Iran.

Research Center of Oils and Fats, Kermanshah University of Medical Sciences, Kermanshah, Iran.

出版信息

Sci Rep. 2024 Jan 2;14(1):161. doi: 10.1038/s41598-023-50664-6.

DOI:10.1038/s41598-023-50664-6
PMID:38168144
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10762099/
Abstract

In the current study, the creation of a chitosan/alginate scaffold hydrogel with and without FeO-NPs or CuO-NPs was studied. From fetal ovine bone marrow mesenchymal stem cells (BM-MSCs) were isolated and cultivated. Their differentiation into osteocyte and adipose cells was investigated. Also, on the scaffolds, cytotoxicity and apoptosis were studied. To investigate the differentiation, treatment groups include: (1) BM-MSCs were plated in DMEM culture medium with high glucose containing 10% FBS and antibiotics (negative control); (2) BM-MSCs were plated in osteogenic differentiation medium (positive control); (3) positive control group + FeO-NPs, (4) positive control group + CuO-NPs; (5) BM-MSCs were plated in osteogenic differentiation medium on chitosan/alginate scaffold; (6) BM-MSCs were plated in osteogenic differentiation medium on chitosan/alginate/FeO-NPs scaffold; and (7) BM-MSCs were plated in osteogenic differentiation medium on chitosan/alginate/CuO-NPs scaffold. Alkaline phosphatase enzyme concentrations, mineralization rate using a calcium kit, and mineralization measurement by alizarin staining quantification were evaluated after 21 days of culture. In addition, qRT-PCR was used to assess the expression of the ALP, ColA, and Runx2 genes. When compared to other treatment groups, the addition of CuO-NPs in the chitosan/alginate hydrogel significantly increased the expression of the ColA and Runx2 genes (p < 0.05). However, there was no significant difference between the chitosan/alginate hydrogel groups containing FeO-NPs and CuO-NPs in the expression of the ALP gene. It appears that the addition of nanoparticles, in particular CuO-NPs, has made the chitosan/alginate scaffold more effective in supporting osteocyte differentiation.

摘要

在目前的研究中，研究了具有和不具有 FeO-NPs 或 CuO-NPs 的壳聚糖/海藻酸钠支架水凝胶的制备。从胎羊骨髓间充质干细胞（BM-MSCs）中分离并培养。研究了它们向成骨细胞和脂肪细胞的分化。此外，还研究了支架上的细胞毒性和细胞凋亡。为了研究分化，处理组包括：（1）将 BM-MSCs 接种在含 10% FBS 和抗生素的高糖 DMEM 培养基中（阴性对照）；（2）将 BM-MSCs 接种在成骨分化培养基中（阳性对照）；（3）阳性对照+FeO-NPs；（4）阳性对照+CuO-NPs；（5）将 BM-MSCs 接种在壳聚糖/海藻酸钠支架上的成骨分化培养基中；（6）将 BM-MSCs 接种在壳聚糖/海藻酸钠/FeO-NPs 支架上的成骨分化培养基中；（7）将 BM-MSCs 接种在壳聚糖/海藻酸钠/CuO-NPs 支架上的成骨分化培养基中。培养 21 天后，评估碱性磷酸酶酶浓度、钙试剂盒测量的矿化率以及茜素红染色定量的矿化测量。此外，使用 qRT-PCR 评估 ALP、ColA 和 Runx2 基因的表达。与其他处理组相比，壳聚糖/海藻酸钠水凝胶中添加 CuO-NPs 显著增加了 ColA 和 Runx2 基因的表达（p<0.05）。然而，在壳聚糖/海藻酸钠水凝胶组中，FeO-NPs 和 CuO-NPs 的添加在 ALP 基因的表达方面没有显著差异。似乎纳米粒子的添加，特别是 CuO-NPs 的添加，使壳聚糖/海藻酸钠支架更有效地支持成骨细胞分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/ca84d74d9eb4/41598_2023_50664_Fig18_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/8eb6c45a8e37/41598_2023_50664_Fig6_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/9f602782c43d/41598_2023_50664_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/f191968074eb/41598_2023_50664_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/b2c93ba962f7/41598_2023_50664_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/f522c8ae95db/41598_2023_50664_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/dd795956b21f/41598_2023_50664_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/9eddf4eddaa0/41598_2023_50664_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/d4e68b975860/41598_2023_50664_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/fc63af01256a/41598_2023_50664_Fig15_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/a55924e48c5f/41598_2023_50664_Fig16_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/c1f979223b72/41598_2023_50664_Fig17_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab70/10762099/ca84d74d9eb4/41598_2023_50664_Fig18_HTML.jpg

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[1]
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[2]
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Cell Biochem Funct. 2022-10

[3]
Preparation and Properties of Iron Nanoparticle-Based Macroporous Scaffolds for Biodegradable Implants.

Materials (Basel). 2022-7-14

[4]
Carboxymethyl chitosan-alginate enhances bone repair effects of magnesium phosphate bone cement by activating the FAK-Wnt pathway.

Bioact Mater. 2022-7-1

[5]
Fabrication, characterization, and optimization of a novel copper-incorporated chitosan/gelatin-based scaffold for bone tissue engineering applications.

Bioimpacts. 2022

[6]
Chitosan nanocarriers for microRNA delivery and detection: A preliminary review with emphasis on cancer.

Carbohydr Polym. 2022-8-15

[7]
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[8]
Copper as Dietary Supplement for Bone Metabolism: A Review.

Nutrients. 2021-6-29

[9]
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Cancers (Basel). 2021-5-5

[10]
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