Sundararaman Aravind, Lee Sang-Seob
Department of Life Science, Kyonggi University, Suwon, 433-760, Republic of Korea.
Antonie Van Leeuwenhoek. 2017 Jan;110(1):45-51. doi: 10.1007/s10482-016-0772-8. Epub 2016 Sep 20.
A novel Gram-negative, non-motile, non-spore forming, facultatively anaerobic and short-rod shaped bacterial strain, designated as 325-5, was isolated from marine sediment obtained from a coastal region in South Korea. Phylogenetic analysis based on the 16S rRNA gene sequence of strain 325-5 showed close similarity to Lutibacter crassostreae (97.8 %). The novel isolate was found to grow optimally at 25 °C and pH 7.0. The major fatty acids identified in the strain were iso C and iso C 3OH, which supports the affiliation of strain 325-5 to the genus Lutibacter. Menaquinone (MK-6) was identified as the respiratory quinone component. The polar lipid profile was found to contain phosphatidylethanolamine and two unidentified lipids. The G+C molar content of strain 325-5 was determined to be 33.1 mol%. DNA-DNA hybridization of strain 325-5 exhibited less than 40 % relatedness to L. crassostreae KCTC 42461. Pigment analysis showed the presence of carotenoid pigments. Based on this polyphasic analysis, we propose that strain 325-5 represents a novel species of the genus Lutibacter, for which the name Lutibacter oceani sp. nov. (type strain 325-5 = JCM 30924 = KEMB 7306-529) is proposed.
从韩国沿海地区采集的海洋沉积物中分离出一株新型革兰氏阴性、无运动性、不形成芽孢、兼性厌氧的短杆状细菌菌株，命名为325-5。基于菌株325-5的16S rRNA基因序列的系统发育分析表明，它与厚壳贻贝卢蒂氏菌（Lutibacter crassostreae）具有密切的相似性（97.8%）。发现该新型分离株在25°C和pH 7.0条件下生长最佳。在该菌株中鉴定出的主要脂肪酸为异C和异C 3OH，这支持了菌株325-5属于卢蒂氏菌属。鉴定出甲基萘醌（MK-6）为呼吸醌成分。发现极性脂质谱包含磷脂酰乙醇胺和两种未鉴定的脂质。菌株325-5的G+C摩尔含量测定为33.1 mol%。菌株325-5与厚壳贻贝卢蒂氏菌KCTC 42461的DNA-DNA杂交显示相关性低于40%。色素分析表明存在类胡萝卜素色素。基于这种多相分析，我们提议菌株325-5代表卢蒂氏菌属的一个新物种，为此提出名称海洋卢蒂氏菌（Lutibacter oceani sp. nov.）（模式菌株325-5 = JCM 30924 = KEMB 7306-529）。
